Real time measures of prestin charge and fluorescence during plasma membrane trafficking reveal sub-tetrameric activity.
Real time measures of prestin charge and fluorescence during plasma membrane trafficking reveal sub-tetrameric activity.
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DOI：
10.1371/journal.pone.0066078
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Santos-Sacchi J
中科院分区：
文献类型：
--
作者：
Bian S;Navaratnam D;Santos-Sacchi J
Prestin(SLC26A5)是一种外毛细胞完整的膜马达蛋白，可驱动耳蜗的扩增，被认为是一种专性四聚体。我们实时研究了YFP-prestin从四环素诱导的细胞系向细胞质膜的输送。在释放温度阻断剂以恢复完整膜蛋白的跨高尔基网络传递后，我们测量了电压钳下的非线性电容(NLC)和膜荧光。Prestin以指数形式递送到质膜，时间常数小于10分钟，电学和荧光法都显示出高度的时间相关性。然而，根据根据荧光或NLC估计的Prestin密度之间的差异，我们得出结论，Prestin的亚四聚体形式对我们的电学和荧光测量有贡献。因此，与以前的观察结果一致，我们发现功能性prestin不是专有的四聚体。
Prestin (SLC26a5) is the outer hair cell integral membrane motor protein that drives cochlear amplification, and has been described as an obligate tetramer. We studied in real time the delivery of YFP-prestin to the plasma membrane of cells from a tetracycline-inducible cell line. Following the release of temperature block to reinstate trans Golgi network delivery of the integral membrane protein, we measured nonlinear capacitance (NLC) and membrane fluorescence during voltage clamp. Prestin was delivered exponentially to the plasma membrane with a time constant of less than 10 minutes, with both electrical and fluorescence methods showing high temporal correlation. However, based on disparity between estimates of prestin density derived from either fluorescence or NLC, we conclude that sub-tetrameric forms of prestin contribute to our electrical and fluorescence measures. Thus, in agreement with previous observations we find that functional prestin is not an obligate tetramer.
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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DOI：
10.1073/pnas.93.15.7661
发表时间：
1996-07-23
影响因子：
11.1
作者：
Andreose, JS;Fumagalli, G;Caplan, MJ
通讯作者：
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