Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane.
Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane.
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DOI：
10.1083/jcb.101.3.949
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发表时间：
1985-09
影响因子：
7.8
通讯作者：
MATLIN, K
中科院分区：
文献类型：
--
作者：
GRIFFITHS, G;PFEIFFER, S;SIMONS, K;MATLIN, K
我们用生物化学、细胞化学和免疫细胞化学方法研究了在20℃运输受阻时水疱性口炎病毒G蛋白在细胞内积累的位置。我们的结果表明，病毒G蛋白在高尔基体堆叠中对酸性磷酸酶染色的那个扁囊内受阻。在20℃时，这个反面扁囊因G蛋白的积累而在结构上发生改变。这种改变的特征是有大面积的被细胞质衣被覆盖的膜芽。这些有衣被的结构有两种——一种能用抗网格蛋白抗体标记，另一种则不能。网格蛋白包被小窝始终不能用抗G抗体标记。当把受感染的细胞升温到32℃时，G蛋白在几分钟内就出现在细胞表面。与此同时，反面扁囊失去了其特有的结构组织。我们进行了双标记实验，将G蛋白定位与辣根过氧化物酶染色相结合，辣根过氧化物酶是通过内吞作用从细胞外介质摄取的。结果表明，反面扁囊不同于内体区室，而且内体不是G蛋白到达细胞表面所经过的必经站。
The intracellular location at which the G protein of vesicular stomatitis virus accumulated when transport was blocked at 20 degrees C has been studied by biochemical, cytochemical, and immunocytochemical methods. Our results indicated that the viral G protein was blocked in that cisterna of the Golgi stack which stained for acid phosphatase. At 20 degrees C this trans cisterna became structurally altered by the accumulation of G protein. This alteration was characterized by extensive areas of membrane buds which were covered by a cytoplasmic coat. These coated structures were of two kinds--those that labeled with anti-clathrin antibodies and those that did not. The clathrin- coated pits consistently did not label with anti-G antibodies. Upon warming infected cells to 32 degrees C, G protein appeared on the surface within minutes. Concomitantly, the trans cisterna lost its characteristic structural organization. Double-labeling experiments were performed in which G protein localization was combined with staining for horseradish peroxidase, which had been taken up from the extracellular medium by endocytosis. The results suggest that the trans cisterna was distinct from the endosome compartment and that the latter was not an obligatory station in the route taken by G protein to the cell surface.
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影响因子：
3.8
作者：
FLAMAND, A
通讯作者：
FLAMAND, A
影响因子：
7.8
作者：
GONATAS, NK;KIM, SU;AVRAMEAS, S
通讯作者：
AVRAMEAS, S
DOI：
10.1073/pnas.78.3.1746
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BERGMANN, JE;TOKUYASU, KT;SINGER, SJ
通讯作者：
SINGER, SJ
影响因子：
7.8
作者：
GRIFFITHS, G;QUINN, P;WARREN, G
通讯作者：
WARREN, G
影响因子：
7.8
作者：
HAND, AR;OLIVER, C
通讯作者：
OLIVER, C