The flexibility and dynamics of the tubules in the endoplasmic reticulum.
The flexibility and dynamics of the tubules in the endoplasmic reticulum.
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DOI：
10.1038/s41598-017-16570-4
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发表时间：
2017-11-28
影响因子：
4.6
通讯作者：
Waigh TA
中科院分区：
文献类型：
--
作者：
Georgiades P;Allan VJ;Wright GD;Woodman PG;Udommai P;Chung MA;Waigh TA
内质网(ER)是真核细胞中的单个细胞器，广泛分布于整个细胞，参与多种细胞功能。在衍射限制和超分辨荧光显微镜(STORM)实验中，使用固定细胞和活细胞(人MRC5肺细胞)，我们确定ER小管的平均持续长度为3.0 3 ± 0.2 4μm，从分析中去掉分支网络连接导致平均持续长度略有增加，为4.71 ± 0.14 μm，并提供了中等长度的尺度依赖关系。肾小管平均半径为44.1 ± 3.2 nm。内质网小管膜的弯曲刚度为10.9 ± 1.2kT(不含分支点的为17.0 ± 1.3kT)。我们研究了活细胞中内质网小管的动态行为，发现内质网小管在拉伸下表现为半弹性纤维。大多数内质网小管在张力作用下经历了平衡的横向波动，而少数内质网小管在运动蛋白的驱动下具有活跃的超扩散运动。因此，细胞以一种明确的方式积极地调节内质网的动态，预计这将反过来影响其许多功能。
The endoplasmic reticulum (ER) is a single organelle in eukaryotic cells that extends throughout the cell and is involved in a large number of cellular functions. Using a combination of fixed and live cells (human MRC5 lung cells) in diffraction limited and super-resolved fluorescence microscopy (STORM) experiments, we determined that the average persistence length of the ER tubules was 3.03 ± 0.24 μm. Removing the branched network junctions from the analysis caused a slight increase in the average persistence length to 4.71 ± 0.14 μm, and provides the tubule’s persistence length with a moderate length scale dependence. The average radius of the tubules was 44.1 ± 3.2 nm. The bending rigidity of the ER tubule membranes was found to be 10.9 ± 1.2 kT (17.0 ± 1.3 kT without branch points). We investigated the dynamic behaviour of ER tubules in live cells, and found that the ER tubules behaved like semi-flexible fibres under tension. The majority of the ER tubules experienced equilibrium transverse fluctuations under tension, whereas a minority number of them had active super-diffusive motions driven by motor proteins. Cells thus actively modulate the dynamics of the ER in a well-defined manner, which is expected in turn to impact on its many functions.
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影响因子：
48
作者：
Dempsey, Graham T.;Vaughan, Joshua C.;Chen, Kok Hao;Bates, Mark;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
11.8
作者：
Eden ER;Sanchez-Heras E;Tsapara A;Sobota A;Levine TP;Futter CE
通讯作者：
Futter CE
DOI：
10.1007/s00249-014-0989-2
发表时间：
2014-12-01
影响因子：
2
作者：
Baroji, Younes F.;Oddershede, Lene B.;Bendix, Poul M.
通讯作者：
Bendix, Poul M.
影响因子：
5.5
作者：
Dobrynin AV;Carrillo JM;Rubinstein M
通讯作者：
Rubinstein M
影响因子：
4.6
作者：
Barooji, Younes F.;Rorvig-Lund, Andreas;Bendix, Poul M.
通讯作者：
Bendix, Poul M.