The life history of Drosophila sperm involves molecular continuity between male and female reproductive tracts.
The life history of Drosophila sperm involves molecular continuity between male and female reproductive tracts.
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DOI：
10.1073/pnas.2119899119
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发表时间：
2022-03-15
影响因子：
11.1
通讯作者：
Dorus S
中科院分区：
文献类型：
--
作者：
McCullough EL;Whittington E;Singh A;Pitnick S;Wolfner MF;Dorus S
在内部受精的物种中，精子在雌性体内度过了生命中重要的一部分。为了研究这段时间精子的生活史，我们在果蝇中使用了半定量蛋白质组学和性别特异性同位素标记来确定雄性和雌性生殖道之间的分子连续性程度，并提供了精子相关蛋白的全球目录。多种精液蛋白和雌性蛋白在交配后立即与精子结合。在精子长期储存后，精液中保留的蛋白质很少，而女性来源的蛋白质则占到交配后精子蛋白质组的五分之一。我们的数据揭示了从男性到女性的分子“传递”，我们假设这是精子与女性相互作用的重要组成部分。精子和女性生殖道（FRT）之间的相互作用对生殖成功至关重要，但人们对其了解甚少。由于精子在FRT内完成其功能成熟，精子的生活史很可能包括从男性到女性的分子“交接”。虽然这种两性间的分子连续性很可能在所有内部受精的物种中广泛存在，但雌性的贡献的身份和程度在很大程度上是未知的。我们将半定量蛋白质组学与性别特异性同位素标记相结合，编录了精子蛋白质组的睾丸后生活史，并确定了男性和frt之间的分子连续性程度。我们发现，黑腹果蝇精子蛋白质组在转移到FRT后发生了实质性的组成变化。多种精液蛋白质最初与精子相关，但在精子储存后，大多数蛋白质变得无法检测到。雌性来源的蛋白质在交配后立即开始与精子结合，在FRT中储存4天后，它们占交配后精子蛋白质组的近20%。与精子相关的雌性来源的蛋白质在与能量代谢相关的过程中富集，这表明在交配和受精之间的长时间内，雌性的贡献支持精子的活力。我们的研究提供了精子蛋白质组动力学的全面表征，并扩展了我们对精子- frt相互作用关键过程的理解。
In species with internal fertilization, sperm spend an important part of their lives within the female. To examine the life history of the sperm during this time, we used semiquantitative proteomics and sex-specific isotopic labeling in fruit flies to determine the extent of molecular continuity between male and female reproductive tracts and provide a global catalog of sperm-associated proteins. Multiple seminal fluid proteins and female proteins associate with sperm immediately after mating. Few seminal fluid proteins remain after long-term sperm storage, whereas female-derived proteins constitute one-fifth of the postmating sperm proteome by then. Our data reveal a molecular “hand-off” from males to females, which we postulate to be an important component of sperm–female interactions. Interactions between sperm and the female reproductive tract (FRT) are critical to reproductive success and yet are poorly understood. Because sperm complete their functional maturation within the FRT, the life history of sperm is likely to include a molecular “hand-off” from males to females. Although such intersexual molecular continuity is likely to be widespread among all internally fertilizing species, the identity and extent of female contributions are largely unknown. We combined semiquantitative proteomics with sex-specific isotopic labeling to catalog the posttesticular life history of the sperm proteome and determine the extent of molecular continuity between male and FRTs. We show that the Drosophila melanogaster sperm proteome undergoes substantial compositional changes after being transferred to the FRT. Multiple seminal fluid proteins initially associate with sperm, but most become undetectable after sperm are stored. Female-derived proteins also begin to associate with sperm immediately after mating, and they comprise nearly 20% of the postmating sperm proteome following 4 d of storage in the FRT. Female-derived proteins that associate with sperm are enriched for processes associated with energy metabolism, suggesting that female contributions support sperm viability during the prolonged period between copulation and fertilization. Our research provides a comprehensive characterization of sperm proteome dynamics and expands our understanding of the critical process of sperm–FRT interactions.
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影响因子：
2
作者：
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通讯作者：
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影响因子：
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