ERF9 of Poncirus trifoliata (L.) Raf. undergoes feedback regulation by ethylene and modulates cold tolerance via regulating a glutathione S-transferase U17 gene.
ERF9 of Poncirus trifoliata (L.) Raf. undergoes feedback regulation by ethylene and modulates cold tolerance via regulating a glutathione S-transferase U17 gene.
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DOI：
10.1111/pbi.13705
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发表时间：
2022-01
影响因子：
13.8
通讯作者：
Liu JH
中科院分区：
文献类型：
--
作者：
Zhang Y;Ming R;Khan M;Wang Y;Dahro B;Xiao W;Li C;Liu JH
植物乙烯响应因子（ERFs）在冷胁迫响应中发挥着重要作用，但这一过程的分子机制尚不清楚。在这项研究中，我们对三叶橙（Poncirus trifoliata (L.)）中的PtrERF9进行了表征。)，一种耐寒植物。PtrERF9在低温下以乙烯依赖的方式上调。过表达PtrERF9显著增强了抗冻能力，当PtrERF9被病毒诱导的基因沉默敲除时，抗冻能力急剧下降。全球转录组分析表明，PtrERF9的沉默导致参与不同生物过程的应激反应基因的大量转录重编程。进一步证实PtrERF9可直接特异性结合谷胱甘肽S转移酶U17 （ptgstu17）和ACC合成酶1 （PtrACS1）启动子。一致地，PtrERF9‐过表达的植株具有更高水平的PtrGSTU17转录物和GST活性，但积累的ROS较少，而沉默的植株则表现出相反的变化。同时，PtrERF9的敲除降低了PtrACS1的表达、ACS活性和ACC含量。然而，PtrERF9在柠檬这种对冷敏感的物种中过表达，对乙烯生物合成的影响可以忽略不计，这是由于PtrERF9及其同源物ClERF9与柠檬ACS1基因启动子（ClACS1）之间的相互作用由于顺式作用元件的突变而受到干扰。综上所述，这些结果表明PtrERF9作用于乙烯信号的下游，并通过调节PtrGSTU17调节ROS稳态，在耐寒性中发挥积极作用。此外，PtrERF9通过激活PtrACS1基因调控乙烯生物合成，形成一个反馈调控回路，加强对其靶基因的转录调控，这可能是三叶Poncirus troliata优异耐寒性的原因之一。
Plant ethylene‐responsive factors (ERFs) play essential roles in cold stress response, but the molecular mechanisms underlying this process remain poorly understood. In this study, we characterized PtrERF9 from trifoliate orange (Poncirus trifoliata (L.) Raf.), a cold‐hardy plant. PtrERF9 was up‐regulated by cold in an ethylene‐dependent manner. Overexpression of PtrERF9 conferred prominently enhanced freezing tolerance, which was drastically impaired when PtrERF9 was knocked down by virus‐induced gene silencing. Global transcriptome profiling indicated that silencing of PtrERF9 resulted in substantial transcriptional reprogramming of stress‐responsive genes involved in different biological processes. PtrERF9 was further verified to directly and specifically bind with the promoters of glutathione S‐transferase U17 (PtrGSTU17) and ACC synthase1 (PtrACS1). Consistently, PtrERF9‐overexpressing plants had higher levels of PtrGSTU17 transcript and GST activity, but accumulated less ROS, whereas the silenced plants showed the opposite changes. Meanwhile, knockdown of PtrERF9 decreased PtrACS1 expression, ACS activity and ACC content. However, overexpression of PtrERF9 in lemon, a cold‐sensitive species, caused negligible alterations of ethylene biosynthesis, which was attributed to perturbed interaction between PtrERF9, along with lemon homologue ClERF9, and the promoter of lemon ACS1 gene (ClACS1) due to mutation of the cis‐acting element. Taken together, these results indicate that PtrERF9 acts downstream of ethylene signalling and functions positively in cold tolerance via modulation of ROS homeostasis by regulating PtrGSTU17. In addition, PtrERF9 regulates ethylene biosynthesis by activating PtrACS1 gene, forming a feedback regulation loop to reinforce the transcriptional regulation of its target genes, which may contribute to the elite cold tolerance of Poncirus trifoliata.
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