Regulation of L-ascorbic acid content in strawberry fruits.
Regulation of L-ascorbic acid content in strawberry fruits.
复制标题
DOI：
10.1093/jxb/err122
复制
发表时间：
2011-08
影响因子：
6.9
通讯作者：
Valpuesta V
中科院分区：
文献类型：
--
作者：
Cruz-Rus E;Amaya I;Sánchez-Sevilla JF;Botella MA;Valpuesta V
植物体内存在多种L-抗坏血酸（阿萨）合成途径，但不同物种、不同器官和不同发育阶段的植物体内各途径对阿萨合成的贡献不同。草莓（Fragaria×ananassa）果实中含有丰富的阿萨。利用D-半乳糖醛酸作为起始底物的途径在成熟果实中是有功能的，但其他途径对阿萨生物合成的贡献尚未研究。半乳糖醛酸还原酶（FaGalUR）、肌醇加氧酶（FaMIOX）和阿萨循环酶单脱氢抗坏血酸还原酶（FaMDHAR）基因的转录与果实成熟过程中阿萨的增加呈正相关。冷藏72 h可使阿萨含量降低30%。在臭氧气氛下，这种减少是15%。臭氧处理增加了FaGalUR，FaMIOX和L-半乳糖-1-磷酸磷酸酶（FaGIPP）基因的表达，L-半乳糖酸-1，4-内酯脱氢酶（FaGLDH）和FAMDHAR基因的转录在臭氧贮藏的果实中高于空气贮藏的果实。从两个草莓品种分离群体中的阿萨含量的分析表明，高的变异性，这并不与任何研究的基因的转录。对不同草莓品种和草莓属不同种的GalUR蛋白的研究表明，GalUR与阿萨含量在大多数情况下存在明显的相关性，但这种相关性并不普遍。三个等位基因被确定在草莓中，但任何序列的影响，阿萨的变异性被消除的等位基因特异性表达的分析。综上所述，这些结果表明，FaGalUR与草莓果实中的其他酶和调节元件共享对阿萨水平的控制。
Plants have several L-ascorbic acid (AsA) biosynthetic pathways, but the contribution of each one to the synthesis of AsA varyies between different species, organs, and developmental stages. Strawberry (Fragaria×ananassa) fruits are rich in AsA. The pathway that uses D-galacturonate as the initial substrate is functional in ripe fruits, but the contribution of other pathways to AsA biosynthesis has not been studied. The transcription of genes encoding biosynthetic enzymes such as D-galacturonate reductase (FaGalUR) and myo-inositol oxygenase (FaMIOX), and the AsA recycling enzyme monodehydroascorbate reductase (FaMDHAR) were positively correlated with the increase in AsA during fruit ripening. Fruit storage for 72 h in a cold room reduced the AsA content by 30%. Under an ozone atmosphere, this reduction was 15%. Ozone treatment increased the expression of the FaGalUR, FaMIOX, and L-galactose-1-phosphate phosphatase (FaGIPP) genes, and transcription of the L-galactono-1,4-lactone dehydrogenase (FaGLDH) and FAMDHAR genes was higher in the ozone-stored than in the air-stored fruits. Analysis of AsA content in a segregating population from two strawberry cultivars showed high variability, which did not correlate with the transcription of any of the genes studied. Study of GalUR protein in diverse cultivars of strawberry and different Fragaria species showed that a correlation between GalUR and AsA content was apparent in most cases, but it was not general. Three alleles were identified in strawberry, but any sequence effect on the AsA variability was eliminated by analysis of the allele-specific expression. Taken together, these results indicate that FaGalUR shares the control of AsA levels with other enzymes and regulatory elements in strawberry fruit.
登录
查看更多内容
影响因子：
6.9
作者：
Ioannidi E;Kalamaki MS;Engineer C;Pateraki I;Alexandrou D;Mellidou I;Giovannonni J;Kanellis AK
通讯作者：
Kanellis AK
DOI：
10.1016/0378-4347(93)80314-t
发表时间：
1993-05-05
期刊：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS
影响因子：
--
作者：
HARAPANHALLI, RS;HOWELL, RW;RAO, DV
通讯作者：
RAO, DV
影响因子：
4.9
作者：
Imai, T;Karita, S;Hirai, M
通讯作者：
Hirai, M
影响因子：
6.4
作者：
Eltayeb, AE;Kawano, N;Tanaka, K
通讯作者：
Tanaka, K
影响因子：
5.2
作者：
Hemavathi;Upadhyaya, Chandrama Prakash;Park, Se Won
通讯作者：
Park, Se Won