Expression profiling of ascorbic acid-related genes during tomato fruit development and ripening and in response to stress conditions.
Expression profiling of ascorbic acid-related genes during tomato fruit development and ripening and in response to stress conditions.
复制标题
DOI：
10.1093/jxb/ern322
复制
发表时间：
2009
影响因子：
6.9
通讯作者：
Kanellis AK
中科院分区：
文献类型：
--
作者：
Ioannidi E;Kalamaki MS;Engineer C;Pateraki I;Alexandrou D;Mellidou I;Giovannonni J;Kanellis AK
L-抗坏血酸（维生素 C 的还原形式）参与多种生物过程，包括病原体防御机制以及植物生长和形态的调节，还充当酶辅助因子和氧化还原状态指示剂。其主要生物功能之一是作为抗氧化剂。 L-抗坏血酸的摄入与预防/减轻包括癌症在内的各种人类疾病有关。为了研究这种重要营养物质在水果中积累的调节，我们对水果发育和成熟过程中参与 L-抗坏血酸生物合成、氧化和再循环的 24 个番茄 (Solanum lycopersicon) 基因的表达进行了表征。与 L-抗坏血酸丰度数据结合起来，结果显示这些基因的表达谱发生了明显变化，表明它们在番茄果实中 L-抗坏血酸积累过程中的节点调节作用。在非生物和收获后应激的背景下进一步研究了这些基因的表达，包括热、冷、伤害、供氧和乙烯的影响。讨论了番茄果实缺氧和缺氧后反应的重要方面。数据表明，L-半乳糖-1-磷酸磷酸酶在调节番茄果实发育和成熟过程中抗坏血酸的积累中发挥重要作用。
L-Ascorbate (the reduced form of vitamin C) participates in diverse biological processes including pathogen defence mechanisms, and the modulation of plant growth and morphology, and also acts as an enzyme cofactor and redox status indicator. One of its chief biological functions is as an antioxidant. L-Ascorbate intake has been implicated in the prevention/alleviation of varied human ailments and diseases including cancer. To study the regulation of accumulation of this important nutraceutical in fruit, the expression of 24 tomato (Solanum lycopersicon) genes involved in the biosynthesis, oxidation, and recycling of L-ascorbate during the development and ripening of fruit have been characterized. Taken together with L-ascorbate abundance data, the results show distinct changes in the expression profiles for these genes, implicating them in nodal regulatory roles during the process of L-ascorbate accumulation in tomato fruit. The expression of these genes was further studied in the context of abiotic and post-harvest stress, including the effects of heat, cold, wounding, oxygen supply, and ethylene. Important aspects of the hypoxic and post-anoxic response in tomato fruit are discussed. The data suggest that L-galactose-1-phosphate phosphatase could play an important role in regulating ascorbic acid accumulation during tomato fruit development and ripening.
登录
查看更多内容
影响因子：
7.2
作者：
Gatzek, S;Wheeler, GL;Smirnoff, N
通讯作者：
Smirnoff, N
影响因子：
7.4
作者：
Davey, MW;Gilot, C;Van Montagu, MC
通讯作者：
Van Montagu, MC
影响因子：
4.3
作者：
Gechev, T;Willekens, H;Minkov, I
通讯作者：
Minkov, I
DOI：
10.1073/pnas.93.18.9970
发表时间：
1996-09-03
影响因子：
11.1
作者：
Conklin, PL;Williams, EH;Last, RL
通讯作者：
Last, RL
影响因子：
6.5
作者：
Badejo, Adebanjo A.;Jeong, Seok T.;Esaka, Muneharu
通讯作者：
Esaka, Muneharu