Inverse association between glutathione peroxidase activity and both selenium-binding protein 1 levels and Gleason score in human prostate tissue.
Inverse association between glutathione peroxidase activity and both selenium-binding protein 1 levels and Gleason score in human prostate tissue.
复制标题
DOI：
10.1002/pros.21506
复制
发表时间：
2012-06-15
期刊：
影响因子：
2.8
通讯作者：
Diamond, Alan M.
中科院分区：
文献类型：
--
作者：
Jerome-Morais, Anita;Wright, Margaret E.;Liu, Rui;Yang, Wancai;Jackson, Matthew I.;Combs, Gerald F., Jr.;Diamond, Alan M.
来自人类流行病学研究、培养的哺乳动物细胞和动物模型的数据支持硒（Se）在前列腺癌预防中的潜在有益作用。此外，含硒蛋白包括谷胱甘肽过氧化物酶（GPx）家族成员和硒结合蛋白1 （SBP1）与癌症风险或发展有关。例如，与非癌组织相比，肿瘤中的SBP1水平通常会降低，而降低的程度与越来越差的临床结果相关。为了利用Gleason评分研究血液和组织硒水平与GPx活性、组织SBP1水平和疾病侵袭性之间的相互关系，我们测量了24名接受根治性前列腺癌治疗的男性的空腹血清和组织学正常前列腺组织中的硒状态生物标志物。使用抗SBP1抗体获得的western blots密度测定结果显示，前列腺组织中GPx酶活性与SBP1水平呈负相关（总体和非裔美国人的部分Spearman相关系数和相应p值分别为- 0.42（0.08）和- 0.53(0.10)），这与之前在培养细胞和小鼠中的观察结果一致。特别令人感兴趣的是组织GPx活性与Gleason评分之间的正相关，这种关系在非裔美国人中具有统计学意义（r=0.67, p=0.02）。这些研究支持了硒和硒蛋白在前列腺癌预防、发展和预后中的作用的持续研究。
Data from human epidemiological studies, cultured mammalian cells, and animal models have supported a potentially beneficial role of selenium (Se) in prostate cancer prevention. In addition, Se-containing proteins including members of the glutathione peroxidase (GPx) family and Selenium Binding Protein 1 (SBP1) have been linked to either cancer risk or development. For example, SBP1 levels are typically reduced in tumors compared to non-cancerous tissue, with the degree of reduction associated with increasingly poor clinical outcome. In order to investigate inter-relationships between blood and tissue Se levels and GPx activity, tissue SBP1 levels, and disease aggressiveness using the Gleason Score, we measured these selenium status biomarkers in fasting serum and histologically normal prostate tissues obtained from 24 men undergoing radical prostatectomy for the treatment of localized prostate cancer. GPx enzyme activity was inversely correlated with SBP1 levels in prostate tissue as determined by densitometry of western blots obtained using anti-SBP1 antibodies (partial Spearman correlation coefficients and corresponding p-values overall and in African-Americans = −0.42 (0.08) and −0.53 (0.10), respectively), which is consistent with previous observations in cultured cells and mice. Of particular interest was the positive correlation between tissue GPx activity and Gleason Score, with this relationship achieving statistical significance among African Americans (r=0.67, p=0.02). . These studies support the continued investigation of the role of Se and selenoproteins in prostate cancer prevention, development and prognosis.
登录
查看更多内容
影响因子：
3.7
作者：
Pohl NM;Tong C;Fang W;Bi X;Li T;Yang W
通讯作者：
Yang W
影响因子：
56.9
作者：
Kryukov, GV;Castellano, S;Gladyshev, VN
通讯作者：
Gladyshev, VN
影响因子：
11.2
作者：
Zhuo, Pin;Goldberg, Marci;Diamond, Alan M.
通讯作者：
Diamond, Alan M.
影响因子：
4.8
作者：
Gouazé, V;Andrieu-Abadie, N;Levade, T
通讯作者：
Levade, T
影响因子：
3
作者：
Zhuo, Pin;Diamond, Alan M.
通讯作者：
Diamond, Alan M.