Speciation analysis of urine iodine by ion-pair reversed-phase liquid chromatography and inductively coupled plasma mass spectrometry
Speciation analysis of urine iodine by ion-pair reversed-phase liquid chromatography and inductively coupled plasma mass spectrometry
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离子对反相液相色谱和电感耦合等离子体质谱法分析尿碘形态
DOI：
10.1039/c4ay00396a
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发表时间：
2014-06
影响因子：
3.1
通讯作者：
Xu, Zigang
中科院分区：
文献类型：
--
作者：
Sun, Jiannan;Cheng, Heyong;Liu, Jinhua;Xu, Zigang
本文介绍了离子对反相液相色谱-电感耦合等离子体质谱法（RP-LC-ICP-MS）在尿液中碘形态分析中的应用。考虑到绿色分析化学和Ar ICP-MS仪器的要求，采用3种水流动相分离碘化物、碘酸盐和5种碘氨基酸（单碘酪氨酸- MIT、二碘酪氨酸- DIT、三碘甲状腺原氨酸- T3、逆三碘甲状腺原氨酸- rT3、甲状腺素- T4）。水流动相由离子对试剂（四丁基氢氧化铵- TBAH）和洗脱液（氯化铵、l -苯丙氨酸或脱氧胆酸）在超纯水中的低浓度组成。由于这些碘化形式之间的保留行为存在巨大差异，采用梯度洗脱模式分别对IO3 -和I -、MIT和DIT、T3、rT3和T4进行了快速分离。用12.5 mm C18保护柱在7 min内完成碘的分离。检测限分别为0.047、0.046、0.057、0.072、0.093、0.094和0.081 μg L−1。通过对四个实际尿样中碘的形态分析，验证了该方法的应用。该方法回收率在93 ~ 106%范围内，重复性好，在环境、食品和生物样品中碘形态的常规分析中具有较大的应用潜力。
This work described the utilization of ion-pair reversed phase liquid chromatography coupled to inductively coupled plasma mass spectrometry (RP-LC-ICP-MS) for iodine speciation analysis in urine. Considering the requirements of green analytical chemistry and the Ar ICP-MS instrument, three aqueous mobile phases were employed for the separation of seven iodine species including iodide, iodate and five iodo amino acids (monoiodotyrosine – MIT, di-iodotyrosine – DIT, tri-iodothyronine – T3, reversed tri-iodothyronine – rT3, and thyroxine – T4). The aqueous mobile phases were composed of an ion-pair reagent (tetrabutylammonium hydroxide – TBAH) and an eluent (ammonium chloride, L-phenylalanine or deoxycholic acid) at low concentrations in ultrapure water. Owing to tremendous difference in retention behavior between these iodinated forms, a gradient elution mode was performed for the rapid separation of IO3− and I−, MIT and DIT, and T3, rT3 and T4, respectively. Iodine species separation was achieved with a 12.5 mm C18 guard column in 7 min. The detection limits for IO3−, I−, MIT, DIT, T3, rT3 and T4 were 0.047, 0.046, 0.057, 0.072, 0.093, 0.094 and 0.081 μg L−1, respectively. Application of the proposed method was demonstrated by the speciation analysis of iodine in four real urine samples. The developed method offered satisfactory recoveries in the 93–106% range and good repeatability, showing great potential in routine analysis of iodine speciation in environmental, food and biological samples.
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影响因子：
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作者：
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