Calcineurin is required for male sex pheromone biosynthesis and female acceptance
Calcineurin is required for male sex pheromone biosynthesis and female acceptance
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男性性信息素生物合成和女性接受需要钙调神经磷酸酶
DOI：
10.1111/imb.12379
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发表时间：
2018-06
影响因子：
2.6
通讯作者：
An Shiheng
中科院分区：
文献类型：
--
作者：
Zhao Wenhui;Li Lihong;Zhang Yaling;Liu Xiaoguang;Wei Jizhen;Xie Yaxiong;Du Mengfang;An Shiheng
鳞翅目昆虫性信息素的生物合成受信息素生物合成激活神经肽（PBAN）的调控。PBAN不仅调节雌性性信息素的合成，而且调节雄性性信息素的合成。先前的研究已经证实，PBAN调节性信息素的生物合成使用Ca 2+作为第二信使在所有检查的物种。然而，Ca 2+的下游信号仍然难以捉摸。本研究在棉铃虫雄蛾发束中发现了Ca 2+的下游信号钙调磷酸酶A（calcineurin A，CNA），命名为HaCNA。序列分析表明，HaCNA开放阅读框全长1488个核苷酸，编码495个氨基酸残基。同源性搜索显示HaCNA与其他昆虫的CNA具有高度的氨基酸同一性。发育和空间表达分析表明，HaCNA的mRNA水平达到峰值后24小时出现，HaCNA的表达是无处不在的所有检查组织。活性分析表明，PBAN激活HaCNA，和Ca 2+抑制剂，Lacl 3，减弱PBAN的影响，通过降低HaCNA活性。药理学抑制剂和RNA干扰介导的敲低试验表明，HaCNA的活性抑制和mRNA水平降低均导致雄性性信息素组分[十八醇和（Z）-11十六醇]的产生和雌性交配接受的有效性显著降低。我们的研究结果表明HaCNA作为PBAN/Ca 2+的下游信号，在PBAN诱导的雄性性信息素生物合成和雌性交配接受中起重要作用。
Lepidoptera sex pheromone biosynthesis is regulated by pheromone biosynthesis activating neuropeptide (PBAN). PBAN regulates not only female sex pheromone biosynthesis but also male sex pheromone biosynthesis. Previous research has confirmed that PBAN regulates sex pheromone biosynthesis using Ca2+ as a secondary messenger in all examined species to date. However, the downstream signal of Ca2+ has remained elusive. In the present study, calcineurin A (CNA), a downstream signal of Ca2+, was discovered in Helicoverpa armigera male hairpencil and named HaCNA. Sequence analysis demonstrated that the open reading frame of HaCNA contains 1488 nucleotides encoding 495 amino acid residues. A homology search revealed that HaCNA shares a high amino acid identity with the CNA of other insects. Developmental and spatial expression analyses revealed that the mRNA levels of HaCNA peaked at 24 h after emergence and that HaCNA expression was ubiquitous in all examined tissues. Activity analysis revealed that PBAN activates HaCNA, and a Ca2+ inhibitor, Lacl3, attenuated the effect of PBAN by decreasing HaCNA activity. Pharmacological inhibitor and RNA interference‐mediated knockdown assays revealed that both activity inhibition and decreased mRNA levels of HaCNA led to a significant decrease in the production of the male sex pheromone components [octadecanol and (Z)‐11 hexadecanol)] and in the efficacy of female mating acceptance. Our results demonstrate that HaCNA acts as downstream signal of PBAN/Ca2+ and plays an important role in PBAN‐induced male sex pheromone biosynthesis and female mating acceptance.
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影响因子：
3.7
作者：
Du M;Yin X;Zhang S;Zhu B;Song Q;An S
通讯作者：
An S
影响因子：
1
作者：
M. Birch;D. Lucas;P. R. White
通讯作者：
M. Birch;D. Lucas;P. R. White
影响因子：
7.6
作者：
Jill M. Sundie;D. Kenrick;Vladas Griskevicius;J. Tybur;K. Vohs;D. Beal
通讯作者：
Jill M. Sundie;D. Kenrick;Vladas Griskevicius;J. Tybur;K. Vohs;D. Beal
影响因子：
4
作者：
Yongping Huang;Shaofu Xu;Xianhan Tang;Zhangwu Zhao;Jia-wei Du
通讯作者：
Yongping Huang;Shaofu Xu;Xianhan Tang;Zhangwu Zhao;Jia-wei Du
影响因子：
64.5
作者：
LIU, J;FARMER, JD;SCHREIBER, SL
通讯作者：
SCHREIBER, SL