Vps3 and Vps8 control integrin trafficking from early to recycling endosomes and regulate integrin-dependent functions.
Vps3 and Vps8 control integrin trafficking from early to recycling endosomes and regulate integrin-dependent functions.
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DOI：
10.1038/s41467-018-03226-8
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发表时间：
2018-02-23
影响因子：
16.6
通讯作者：
Klumperman J
中科院分区：
文献类型：
--
作者：
Jonker CTH;Galmes R;Veenendaal T;Ten Brink C;van der Welle REN;Liv N;de Rooij J;Peden AA;van der Sluijs P;Margadant C;Klumperman J
再循环内体维持质膜稳态，对细胞极性、迁移和胞质分裂很重要。然而，驱动内吞再循环的分子机制在很大程度上仍不清楚。CORVET复合物是早期内体之间融合所需的多亚基系链。在这里，我们表明，CORVET特异性亚基Vps 3和Vps 8也调节囊泡运输从早期到回收内体。Vps 3和Vps 8定位于Rab 4阳性再循环囊泡，并与Rab 11阳性再循环内体上的CHEV 1复合物共定位。Vps 3或Vps 8的消耗不影响转铁蛋白再循环，但延迟了内化的整合素向再循环内体的递送及其随后返回质膜。因此，Vps 3/8缺失导致整合素依赖性细胞粘附和扩散、粘着斑形成和细胞迁移的缺陷。这些数据揭示了Vps 3和Vps 8在对整合素运输重要的专门再循环途径中的作用。CORVET复合物是调节早期内体之间融合的多亚基复合物。在这里，作者表明，CORVET亚基Vps 3和Vps 8也调节囊泡运输从早期到再循环内体，并需要回收整合素到质膜。
Recycling endosomes maintain plasma membrane homeostasis and are important for cell polarity, migration, and cytokinesis. Yet, the molecular machineries that drive endocytic recycling remain largely unclear. The CORVET complex is a multi-subunit tether required for fusion between early endosomes. Here we show that the CORVET-specific subunits Vps3 and Vps8 also regulate vesicular transport from early to recycling endosomes. Vps3 and Vps8 localise to Rab4-positive recycling vesicles and co-localise with the CHEVI complex on Rab11-positive recycling endosomes. Depletion of Vps3 or Vps8 does not affect transferrin recycling, but delays the delivery of internalised integrins to recycling endosomes and their subsequent return to the plasma membrane. Consequently, Vps3/8 depletion results in defects in integrin-dependent cell adhesion and spreading, focal adhesion formation, and cell migration. These data reveal a role for Vps3 and Vps8 in a specialised recycling pathway important for integrin trafficking. The CORVET complex is a multi-subunit complex that regulates fusion between early endosomes. Here the authors show that the CORVET subunits Vps3 and Vps8 also regulate vesicular transport from early to recycling endosomes and are required for recycling integrins to the plasma membrane.
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DOI：
10.1111/j.1600-0854.2012.01327.x
发表时间：
2012-04
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Arjonen A;Alanko J;Veltel S;Ivaska J
通讯作者：
Ivaska J
影响因子：
21.3
作者：
McNally KE;Faulkner R;Steinberg F;Gallon M;Ghai R;Pim D;Langton P;Pearson N;Danson CM;Nägele H;Morris LL;Singla A;Overlee BL;Heesom KJ;Sessions R;Banks L;Collins BM;Berger I;Billadeau DD;Burstein E;Cullen PJ
通讯作者：
Cullen PJ
影响因子：
30.8
作者：
Cullinane AR;Straatman-Iwanowska A;Zaucker A;Wakabayashi Y;Bruce CK;Luo G;Rahman F;Gürakan F;Utine E;Ozkan TB;Denecke J;Vukovic J;Di Rocco M;Mandel H;Cangul H;Matthews RP;Thomas SG;Rappoport JZ;Arias IM;Wolburg H;Knisely AS;Kelly DA;Müller F;Maher ER;Gissen P
通讯作者：
Gissen P
影响因子：
4
作者：
De Franceschi N;Hamidi H;Alanko J;Sahgal P;Ivaska J
通讯作者：
Ivaska J
影响因子：
15.9
作者：
Bayram, Yavuz;Karaca, Ender;Lupski, James R.
通讯作者：
Lupski, James R.