A protocol to use Drosophila melanogaster larvae to model human glioblastoma.
A protocol to use Drosophila melanogaster larvae to model human glioblastoma.
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DOI：
10.1016/j.xpro.2022.101609
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发表时间：
2022-09-16
期刊：
影响因子：
--
通讯作者：
Read, Renee D.
中科院分区：
文献类型：
--
作者：
Saborio, Julia G.;Young, Elizabeth E.;Chen, Alexander S.;Read, Renee D.
该方案描述了我们在黑腹果蝇中为胶质母细胞瘤（GBM）开发的一种遗传模型系统，可用于探究突变基因通路的致病表型效应，并确定具有这些突变的肿瘤的潜在治疗靶点。我们介绍了在果蝇幼虫中创建神经胶质瘤脑肿瘤所需的遗传方案和实验步骤。我们还提供了在该模型中操作基因以及对肿瘤幼虫大脑进行固定、免疫染色和成像的步骤。
有关该方案的使用和实施的完整细节，请参考……
使用UAS - Gal4系统在黑腹果蝇中创建胶质母细胞瘤的遗传模型
在肿瘤性神经胶质细胞中测试感兴趣基因的RNA干扰、过表达或突变变体
对肿瘤大脑进行免疫染色和成像，定性和定量分析数据
在完整的果蝇神经系统中探究遗传修饰因子和突变的影响
出版商注：进行任何实验方案都需要遵守当地机构的实验室安全和伦理准则。
该方案描述了我们在黑腹果蝇中为胶质母细胞瘤（GBM）开发的一种遗传模型系统，可用于探究突变基因通路的致病表型效应，并确定具有这些突变的肿瘤的潜在治疗靶点。我们介绍了在果蝇幼虫中创建神经胶质瘤脑肿瘤所需的遗传方案和实验步骤。我们还提供了在该模型中操作基因以及对肿瘤幼虫大脑进行固定、免疫染色和成像的步骤。
This protocol describes a genetic model system we developed for glioblastoma (GBM) in Drosophila melanogaster, which can be used to explore the pathogenic phenotypic effects of mutated genetic pathways and to identify potential therapeutic targets for tumors with these mutations. We present genetic schemes and experimental steps needed to create neoplastic glial brain tumors in larval Drosophila. We also provide steps to manipulate genes in this model and to perform brain fixation, immunostaining, and imaging of neoplastic larval brains. For complete details on the use and execution of this protocol, please refer to. Create genetic models of glioblastoma in Drosophila melanogaster using UAS-Gal4 systems Test RNAi, overexpression, or mutant variants of genes of interest in neoplastic glia Immunostain and image neoplastic brains, analyze data qualitatively and quantitatively Explore effects of genetic modifiers and mutations in intact Drosophila nervous system Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. This protocol describes a genetic model system we developed for glioblastoma (GBM) in Drosophila melanogaster, which can be used to explore the pathogenic phenotypic effects of mutated genetic pathways and to identify potential therapeutic targets for tumors with these mutations. We present genetic schemes and experimental steps needed to create neoplastic glial brain tumors in larval Drosophila. We also provide steps to manipulate genes in this model and to perform brain fixation, immunostaining, and imaging of neoplastic larval brains.
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影响因子：
16.6
作者：
Lemon WC;Pulver SR;Höckendorf B;McDole K;Branson K;Freeman J;Keller PJ
通讯作者：
Keller PJ
DOI：
10.1007/978-3-030-23629-8_12
发表时间：
2019-01-01
期刊：
DROSOPHILA MODEL IN CANCER
影响因子：
--
作者：
Chen, Alexander S.;Read, Renee D.
通讯作者：
Read, Renee D.
影响因子：
1.2
作者：
Karabasheva, Darya;Smyth, Jeremy T.
通讯作者：
Smyth, Jeremy T.
影响因子：
3
作者：
Kriston-Vizi, Janos;Thong, Ng Wee;Wasser, Martin
通讯作者：
Wasser, Martin
影响因子：
2.7
作者：
Omoto, Jaison Jiro;Yogi, Puja;Hartenstein, Volker
通讯作者：
Hartenstein, Volker