Adult Rat Odontoblasts Lack Noxious Thermal Sensitivity
Adult Rat Odontoblasts Lack Noxious Thermal Sensitivity
复制标题
成年大鼠成牙本质细胞缺乏有害热敏感性
DOI：
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Seog Bae Oh
中科院分区：
文献类型：
--
作者：
K. Yeon;G. Chung;M. S. Shin;S. J. Jung;J. Kim;Seog Bae Oh
牙本质过敏症是牙科临床常见的症状，但其细胞和分子机制尚不清楚。我们假设成牙本质细胞通过表达温度敏感分子来检测有害的热刺激，并研究了温度激活的TRP通道（热TRP通道）是否介导成牙本质细胞的温度敏感。通过单细胞RT-PCR，在急性分离的成年大鼠成牙本质细胞中显示牙本质涎磷蛋白和胶原酶1型的mRNA表达，而TRPV 1、TRPV 2、TRPM 8和TRPA 1未检测到。应用42°C和12°C的有害温度以及辣椒素、薄荷醇和icilin（热TRP通道的激动剂）未能增加细胞内钙浓度。免疫组化结果显示TRPV 1无表达。因此，成牙本质细胞不太可能通过热TRP通道作为牙齿中的热传感器。
Dentin hypersensitivity is a common symptom treated in the dental clinic, yet the underlying cellular and molecular mechanisms are not clear. We hypothesized that odontoblasts detect noxious thermal stimuli by expressing temperature-sensing molecules, and investigated whether temperature-activated TRP channels (thermo-TRP channels), which are known to initiate temperature signaling, mediate temperature sensing in odontoblasts. mRNA expression of dentin sialophosphoprotein and collagenase type 1, odontoblast-specific proteins, was shown in acutely isolated adult rat odontoblasts by single-cell RT-PCR, while TRPV1, TRPV2, TRPM8, and TRPA1 were not detected. Application of noxious temperatures of 42°C and 12°C, as well as capsaicin, menthol, and icilin, agonists of thermo-TRP channels, failed to increase intracellular calcium concentration. Immunohistochemical study also revealed no expression of TRPV1. Thus, it is unlikely that odontoblasts serve as thermal sensors in teeth via thermo-TRP channels.
影响因子：
3.9
作者：
Ambudkar, I. S.
通讯作者：
Ambudkar, I. S.
DOI：
10.1016/s0021-9258(17)41916-8
发表时间：
1994-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Helena H. Ritchie;Hui Hou;A. Veis;William T. Butler
通讯作者：
Helena H. Ritchie;Hui Hou;A. Veis;William T. Butler