Genetic characterization of Plectorhinchus mediterraneus yields important clues about genome organization and evolution of multigene families.
Genetic characterization of Plectorhinchus mediterraneus yields important clues about genome organization and evolution of multigene families.
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DOI：
10.1186/1471-2156-13-33
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发表时间：
2012-04-30
期刊：
影响因子：
2.9
通讯作者：
Rebordinos L
中科院分区：
文献类型：
--
作者：
Merlo MA;Pacchiarini T;Portela-Bens S;Cross I;Manchado M;Rebordinos L
分子和细胞遗传学标记在鱼类的性状鉴定、物种遗传学和进化研究中具有重要的应用价值。多基因家族已被证明是更好地了解鱼类物种的变异性，组织和进化的良好标记。从分子和细胞遗传学水平对地中海胡椒鲷（Plectorhinchusmediameus）的3个不同的串联重复基因家族（45 SrDNA、5SrDNA和U2 snDNA）进行了研究，以阐明这些多基因家族的分类和进化，并与该家族其他物种进行比较。获得了四种不同类型的5S rDNA，其中两种显示出与拉贾的高度同源性，并对水平转移事件及其对5S rDNA组织和进化的影响进行了讨论。其他两种类型不像任何其他物种，但在其中一个推定的tRNA衍生的SINE是第一次观察到的，这可能对5S rDNA的进化有影响。ITS-1序列与异属种的亲缘关系较近，因此建议对该科的系统进行修订。在对U2 snDNA的分析中，我们能够证实U2 snDNA和U 5 snDNA在同一串联阵列中连接，这对追踪进化线有意义。该种的核型由2n = 48条近端着丝粒染色体组成，三个多基因科分别位于不同的染色体对上，从而提供了三种不同的染色体标记。新的数据可以从结果中提取：一个假定的水平转移事件，一个可能的tRNA衍生的SINE连接到四个5S rDNA类型之一的特点，和U2和U 5 snDNA之间的联系。此外，还提出了修订的Haemulidae科的分类，并获得了三个细胞遗传学标记。其中一些结果以前在任何其他鱼类中都没有描述过。本研究为多基因家族的基因组结构和进化提供了新的线索。
Molecular and cytogenetic markers are of great use for to fish characterization, identification, phylogenetics and evolution. Multigene families have proven to be good markers for a better understanding of the variability, organization and evolution of fish species. Three different tandemly-repeated gene families (45S rDNA, 5S rDNA and U2 snDNA) have been studied in Plectorhinchus mediterraneus (Teleostei: Haemulidae), at both molecular and cytogenetic level, to elucidate the taxonomy and evolution of these multigene families, as well as for comparative purposes with other species of the family. Four different types of 5S rDNA were obtained; two of them showed a high homology with that of Raja asterias, and the putative implication of a horizontal transfer event and its consequences for the organization and evolution of the 5S rDNA have been discussed. The other two types do not resemble any other species, but in one of them a putative tRNA-derived SINE was observed for the first time, which could have implications in the evolution of the 5S rDNA. The ITS-1 sequence was more related to a species of another different genus than to that of the same genus, therefore a revision of the Hamulidae family systematic has been proposed. In the analysis of the U2 snDNA, we were able to corroborate that U2 snDNA and U5 snDNA were linked in the same tandem array, and this has interest for tracing evolutionary lines. The karyotype of the species was composed of 2n = 48 acrocentric chromosomes, and each of the three multigene families were located in different chromosome pairs, thus providing three different chromosomal markers. Novel data can be extracted from the results: a putative event of horizontal transfer, a possible tRNA-derived SINE linked to one of the four 5S rDNA types characterized, and a linkage between U2 and U5 snDNA. In addition, a revision of the taxonomy of the Haemulidae family has been suggested, and three cytogenetic markers have been obtained. Some of these results have not been described before in any other fish species. New clues about the genome organization and evolution of the multigene families are offered in this study.
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