Arabidopsis TCP4 transcription factor inhibits high temperature-induced homeotic conversion of ovules.

Arabidopsis TCP4 transcription factor inhibits high temperature-induced homeotic conversion of ovules.
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DOI:
10.1038/s41467-023-41416-1
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发表时间:
2023-09-13
影响因子:
16.6
通讯作者:
Qin, Genji
Qin, Genji
中科院分区:
综合性期刊1区
文献类型:
--
作者:
Lan, Jingqiu;Wang, Ning;Wang, Yutao;Jiang, Yidan;Yu, Hao;Cao, Xiaofeng;Qin, Genji

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全球变暖引起的异常高温威胁着植物的生存和粮食安全,但其对植物器官特性的影响尚不清楚。在这里,我们证明了第二类teosinte分支1/CycloidEA/PCF(TCP)转录因子在高温下冗余地保护胚珠的特性。Tcp2/3/4/5/10/13/17/24/1/12/18/16(TcpDUO)突变体表现出HT诱导的胚珠向心皮结构的转化。TCP4在tcpDUO中的表达是胚珠同一性转换的补充。TCP4与AGAMOUS(AG)、SEPALLATA3(SEP3)和同源结构域转录因子BELL1(BEL1)相互作用,加强BEL1与AG-SEP3的结合。TcpDUO突变体与bel1和胚珠识别基因种子棒(Stk)突变体stk在tcpDUO bel1和tcpDUO StK上有协同作用。我们的发现揭示了第二类TCP在高温下维持胚珠配合性的关键作用,并阐明了内部因素和环境温度综合决定胚珠配合性的分子机制。全球变暖威胁着粮食安全和植物生存。本文证明了第二类TCP转录因子在确定胚珠的特性和抑制高温下胚珠向心皮的转化中起着关键作用。
Abnormal high temperature (HT) caused by global warming threatens plant survival and food security, but the effects of HT on plant organ identity are elusive. Here, we show that Class II TEOSINTE BRANCHED 1/CYCLOIDEA/ PCF (TCP) transcription factors redundantly protect ovule identity under HT. The duodecuple tcp2/3/4/5/10/13/17/24/1/12/18/16 (tcpDUO) mutant displays HT-induced ovule conversion into carpelloid structures. Expression of TCP4 in tcpDUO complements the ovule identity conversion. TCP4 interacts with AGAMOUS (AG), SEPALLATA3 (SEP3), and the homeodomain transcription factor BELL1 (BEL1) to strengthen the association of BEL1 with AG-SEP3. The tcpDUO mutant synergistically interacts with bel1 and the ovule identity gene seedstick (STK) mutant stk in tcpDUO bel1 and tcpDUO stk. Our findings reveal the critical roles of Class II TCPs in maintaining ovule identity under HT and shed light on the molecular mechanisms by which ovule identity is determined by the integration of internal factors and environmental temperature. Global warming threatens food security and plant survival. This paper demonstrates that Class II TCP transcription factors are critical for specifying ovule identity and inhibiting the conversion of ovules into carpelloids under high temperature.
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