A transcriptional complex of NGATHA and bHLH transcription factors directs stigma development in Arabidopsis.

A transcriptional complex of NGATHA and bHLH transcription factors directs stigma development in Arabidopsis.
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DOI:
10.1093/plcell/koab236
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发表时间:
2021-12-03
期刊:
The Plant cell
影响因子:
--
通讯作者:
Ferrándiz C
Ferrándiz C
中科院分区:
其他
文献类型:
--
作者:
Ballester P;Martínez-Godoy MA;Ezquerro M;Navarrete-Gómez M;Trigueros M;Rodríguez-Concepción M;Ferrándiz C

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柱头是被子植物特有的组织,对授粉至关重要。在过去的二十年中,已经确定了几个在拟南芥柱头发育中起关键作用的转录因子。然而,迄今为止,遗传分析还无法揭示这些转录因子之间的精确调控相互作用,也无法揭示它们在不同时空域中的选择性作用的分子基础。在这里,我们发现NGATHA (NGA)和HECATE (HEC)转录因子参与不同的发育过程,但都是柱头发育所必需的,它们需要彼此发挥这一功能。这种关系可能是由它们在顶端雌蕊中的物理相互作用介导的。NGA/HEC转录因子随后上调INDEHISCENT (IND)和SPATULA,并且对于IND与一些靶标结合以实现柱头分化是必不可少的。我们的研究结果支持一种非分层调控情景,其中不同转录因子的组合作用为其发育输出提供了精细的时间和空间特异性。拟南芥的柱头发育需要形成不同的高阶转录因子复合物,这些复合物的复杂性不断增加。
The stigma is an angiosperm-specific tissue that is essential for pollination. In the last two decades, several transcription factors with key roles in stigma development in Arabidopsis thaliana have been identified. However, genetic analyses have thus far been unable to unravel the precise regulatory interactions among these transcription factors or the molecular basis for their selective roles in different spatial and temporal domains. Here, we show that the NGATHA (NGA) and HECATE (HEC) transcription factors, which are involved in different developmental processes but are both essential for stigma development, require each other to perform this function. This relationship is likely mediated by their physical interaction in the apical gynoecium. NGA/HEC transcription factors subsequently upregulate INDEHISCENT (IND) and SPATULA and are indispensable for the binding of IND to some of its targets to allow stigma differentiation. Our findings support a nonhierarchical regulatory scenario in which the combinatorial action of different transcription factors provides exquisite temporal and spatial specificity of their developmental outputs. Stigma development in Arabidopsis requires the formation of different higher order transcription factor complexes of increasing complexity in a sequential manner.
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