Adhesion molecule periplakin is involved in cellular movement and attachment in pharyngeal squamous cancer cells.
Adhesion molecule periplakin is involved in cellular movement and attachment in pharyngeal squamous cancer cells.
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DOI：
10.1186/1471-2121-12-41
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发表时间：
2011-09-27
期刊：
影响因子：
--
通讯作者：
Nomura F
中科院分区：
文献类型：
--
作者：
Tonoike Y;Matsushita K;Tomonaga T;Katada K;Tanaka N;Shimada H;Nakatani Y;Okamoto Y;Nomura F
我们以前报道，periplakin（PPL）是下调人食管癌组织相比，相邻的非癌上皮。因此，PPL可能是检测早期食管癌和评估肿瘤进展的有用标志物，但在这一领域仍有很大的未知数。为了研究PPL参与癌发生、肿瘤进展、细胞运动或附着活性，将针对PPL的siRNA转染到咽鳞状细胞癌细胞系中，并检查它们对细胞行为的影响。PPL敲除似乎降低了肿瘤细胞生长以及附着于培养皿的细胞中的G2/M期积累。然而，细胞生长抑制的程度，评估的细胞数量附着到培养皿，是太独特的，不能只解释细胞周期延迟。重要的是，PPL敲低抑制了细胞运动和附着到培养皿，伴随着pAktSer 473磷酸化的降低。此外，LY 294002，一种PI 3 K抑制剂，使pAktSer 473去磷酸化，显著抑制D562细胞迁移。因此，PPL可能至少部分地通过PI 3 K/Akt轴参与细胞运动。PPL敲低与细胞运动和附着活性降低有关，与PI 3 K/Akt轴抑制有关，而不是咽癌细胞的恶性进展。
We previously reported that periplakin (PPL) is downregulated in human esophageal cancer tissues compared to the adjacent non-cancer epithelium. Thus PPL could be a useful marker for detection of early esophageal cancer and evaluation of tumor progression, but largely remains unknown in this field. To investigate PPL involvement in carcinogenesis, tumor progression, cellular movement or attachment activity, siRNAs against PPL were transfected into pharyngeal squamous cancer cell lines and their effects on cellular behaviours were examined. PPL knockdown appeared to decrease tumor cell growth together with G2/M phase accumulation in cells attached to a culture dish. However, the extent of cell growth suppression, evaluated by the number of cells attached to the culture dish, was too distinctive to be explained only by cell cycle delay. Importantly, PPL knockdown suppressed cellular movement and attachment to the culture dish accompanied by decreased pAktSer473 phosphorylation. Additionally, LY294002, a PI3K inhibitor that dephosphorylates pAktSer473, significantly suppressed D562 cell migration. Thus PPL potentially engages in cellular movement al least partly via the PI3K/Akt axis. PPL knockdown is related to reduced cellular movement and attachment activity in association with PI3K/Akt axis suppression, rather than malignant progression in pharyngeal cancer cells.
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影响因子：
11.2
作者：
Matsushita, K;Tomonaga, T;Ochiai, T
通讯作者：
Ochiai, T
影响因子：
3.4
作者：
Hatakeyama, Hiromitsu;Kondo, Tadashi;Hirohashi, Setsuo
通讯作者：
Hirohashi, Setsuo
影响因子：
4.8
作者：
Francke, Felix;Ward, Richard J.;Baechner, Dietmar
通讯作者：
Baechner, Dietmar
影响因子：
4.8
作者：
Kajimoto, T;Shirai, Y;Saito, N
通讯作者：
Saito, N
影响因子：
4
作者：
Bhattacharya R;Gonzalez AM;Debiase PJ;Trejo HE;Goldman RD;Flitney FW;Jones JC
通讯作者：
Jones JC