Regulation of desmocollin transcription in mouse preimplantation embryos.
Regulation of desmocollin transcription in mouse preimplantation embryos.
复制标题
小鼠植入前胚胎中桥粒胶蛋白转录的调节。
作者：
Jane E. Collins;Jo E. Lorimer;D. R. Garrod;S. Pidsley;R. Buxton;Tom P. Fleming
对小鼠胚胎发生过程中形成的第一批桥粒生物发生的分子机制进行了研究。对逆转录酶-cDNA扩增程序进行了灵敏的修改，用来检测桥粒粘附性钙粘附素、桥粒粘附素和桥粒粘附素的转录本。对扩增产物进行测序证实，DSC2基因的两个剪接变异体a和b是协同转录的。转录本在未受精卵和卵丘细胞中被鉴定出来，直到8-细胞早期的卵裂阶段，在致密的8-细胞胚胎中从未被检测到，但在16-细胞桑拿期或极早期(约32-细胞)的囊胚中再次明显。转录检测的这两个阶段表明DSC2是由母体和胚胎基因组编码的。以前，我们已经证明在卵子和卵裂阶段不能检测到桥粒粘连蛋白的合成，但在胚泡早期开始合成，此时桥粒粘连蛋白定位于新生桥粒的组装，并似乎调节新生桥粒的组装，这些桥粒形成于滋养外胚层，但不存在于内细胞团(Fleming，T.P.，Garrod，D.R.和Elsmore，A.J.(1991)，Development 112,527-539)。因此，母体的DSC2mRNA没有被翻译，推测在完全降解之前由卵裂球遗传。然而，我们的结果表明，胚胎DSC2转录的启动调节桥粒蛋白的表达，从而调节桥粒的形成。此外，来自胚泡单细胞分析的数据表明，胚胎DSC2转录是滋养外胚层特有的。抑制E-钙粘附素介导的细胞-细胞黏附不影响DSC2胚胎转录和蛋白表达的时间。然而，内细胞团的分离和培养诱导了DSC2mRNA和蛋白的检测增加。综上所述，这些结果表明，无接触细胞表面的存在激活了小鼠早期胚胎中DSC2的转录。
The molecular mechanisms regulating the biogenesis of the first desmosomes to form during mouse embryogenesis have been studied. A sensitive modification of a reverse transcriptase-cDNA amplification procedure has been used to detect transcripts of the desmosomal adhesive cadherin, desmocollin. Sequencing of cDNA amplification products confirmed that two splice variants, a and b, of the DSC2 gene are transcribed coordinately. Transcripts were identified in unfertilized eggs and cumulus cells and in cleavage stages up to the early 8-cell stage, were never detected in compact 8-cell embryos, but were evident again either from the 16-cell morula or very early blastocyst (approx 32-cells) stages onwards. These two phases of transcript detection indicate DSC2 is encoded by maternal and embryonic genomes. Previously, we have shown that desmocollin protein synthesis is undetectable in eggs and cleavage stages but initiates at the early blastocyst stage when desmocollin localises at, and appears to regulate assembly of, nascent desmosomes that form in the trophectoderm but not in the inner cell mass (Fleming, T. P., Garrod, D. R. and Elsmore, A. J. (1991), Development 112, 527-539). Maternal DSC2 mRNA is therefore not translated and presumably is inherited by blastomeres before complete degradation. Our results suggest, however, that initiation of embryonic DSC2 transcription regulates desmocollin protein expression and thereby desmosome formation. Moreover, data from blastocyst single cell analyses suggest that embryonic DSC2 transcription is specific to the trophectoderm lineage. Inhibition of E-cadherin-mediated cell-cell adhesion did not influence the timing of DSC2 embryonic transcription and protein expression. However, isolation and culture of inner cell masses induced an increase in the amount of DSC2 mRNA and protein detected. Taken together, these results suggest that the presence of a contact-free cell surface activates DSC2 transcription in the mouse early embryo.
影响因子：
10.5
作者：
LATHAM, KE;DOHERTY, AS;SCHULTZ, RM
通讯作者：
SCHULTZ, RM
兔珠蛋白基因簇 psi zeta-alpha 1-psi alpha 的分离和核苷酸序列。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cheng,JF;Raid,L;Hardison,RC
通讯作者：
Hardison,RC
针对 3 非编码区的反义 RNA 可防止小鼠卵母细胞中休眠的 mRNA 激活。
DOI：
10.1126/science.2456615
发表时间：
1988
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Strickland,S;Huarte,J;Belin,D;Vassalli,A;Rickles,RJ;Vassalli,JD
通讯作者：
Vassalli,JD