Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system.
Evolutionary dynamics of methicillin-resistant Staphylococcus aureus within a healthcare system.
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DOI：
10.1186/s13059-015-0643-z
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发表时间：
2015-04-23
期刊：
影响因子：
12.3
通讯作者：
Holden MT
中科院分区：
文献类型：
--
作者：
Hsu LY;Harris SR;Chlebowicz MA;Lindsay JA;Koh TH;Krishnan P;Tan TY;Hon PY;Grubb WB;Bentley SD;Parkhill J;Peacock SJ;Holden MT
在过去的十年里，几个国家已经看到流行的多重耐药的医疗保健相关的耐甲氧西林金黄色葡萄球菌(MRSA)逐渐被对抗生素治疗更敏感的克隆所取代。一个例子是新加坡，自20世纪80年代中期开始对MRSA进行分子分析以来，占主导地位的克隆MRSA ST239已被属于EMRSA-15的ST22分离株所取代，EMRSA-15是最近出现的一种源自欧洲的大流行谱系。我们利用全基因组测序的方法，对新加坡医院中MRSA的种群结构进行了长达30年的调查。应用贝叶斯系统发生学方法，我们报告了在ST22引入之前，新加坡的ST239 MRSA群体来自于来自周围地区的多次引进；它经常在医疗系统内转移，导致医院群体的异质性。随着ST22在本世纪初的引入，这种克隆病毒在新加坡的医院中迅速传播，取代了流行的ST239人群。合并分析表明，虽然ST239的遗传多样性最初随着ST22变得更加显性而降低，但从2007年开始ST239的遗传多样性又开始增加，这与ST239亚克隆的出现无关。对现存的ST239群体的附属基因组的比较基因组分析发现，精氨酸分解代谢活动元件多次出现，从而将与增强皮肤定殖性相关的基因引入该群体。我们的结果清楚地表明，除了临床实践和抗生素的使用外，克隆之间的竞争也在推动医院内病原菌种群的进化方面发挥着重要作用。本文的在线版本(doi：10.1186/s13059-0150643-z)包含补充材料，授权用户可以使用。
In the past decade, several countries have seen gradual replacement of endemic multi-resistant healthcare-associated methicillin-resistant Staphylococcus aureus (MRSA) with clones that are more susceptible to antibiotic treatment. One example is Singapore, where MRSA ST239, the dominant clone since molecular profiling of MRSA began in the mid-1980s, has been replaced by ST22 isolates belonging to EMRSA-15, a recently emerged pandemic lineage originating from Europe. We investigated the population structure of MRSA in Singaporean hospitals spanning three decades, using whole genome sequencing. Applying Bayesian phylogenetic methods we report that prior to the introduction of ST22, the ST239 MRSA population in Singapore originated from multiple introductions from the surrounding region; it was frequently transferred within the healthcare system resulting in a heterogeneous hospital population. Following the introduction of ST22 around the beginning of the millennium, this clone spread rapidly through Singaporean hospitals, supplanting the endemic ST239 population. Coalescent analysis revealed that although the genetic diversity of ST239 initially decreased as ST22 became more dominant, from 2007 onwards the genetic diversity of ST239 began to increase once more, which was not associated with the emergence of a sub-clone of ST239. Comparative genomic analysis of the accessory genome of the extant ST239 population identified that the Arginine Catabolic Mobile Element arose multiple times, thereby introducing genes associated with enhanced skin colonization into this population. Our results clearly demonstrate that, alongside clinical practice and antibiotic usage, competition between clones also has an important role in driving the evolution of nosocomial pathogen populations. The online version of this article (doi:10.1186/s13059-015-0643-z) contains supplementary material, which is available to authorized users.
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影响因子：
3
作者：
Hsu, Li-Yang;Loomba-Chlebicka, Nichi;Koh, Tse-Hsien
通讯作者：
Koh, Tse-Hsien
影响因子：
4.5
作者：
Cosgrove, SE;Qi, YL;Carmeli, Y
通讯作者：
Carmeli, Y
DOI：
10.1126/science.1182395
发表时间：
2010-01-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Harris SR;Feil EJ;Holden MT;Quail MA;Nickerson EK;Chantratita N;Gardete S;Tavares A;Day N;Lindsay JA;Edgeworth JD;de Lencastre H;Parkhill J;Peacock SJ;Bentley SD
通讯作者：
Bentley SD
影响因子：
7
作者：
Holden MT;Hsu LY;Kurt K;Weinert LA;Mather AE;Harris SR;Strommenger B;Layer F;Witte W;de Lencastre H;Skov R;Westh H;Zemlicková H;Coombs G;Kearns AM;Hill RL;Edgeworth J;Gould I;Gant V;Cooke J;Edwards GF;McAdam PR;Templeton KE;McCann A;Zhou Z;Castillo-Ramírez S;Feil EJ;Hudson LO;Enright MC;Balloux F;Aanensen DM;Spratt BG;Fitzgerald JR;Parkhill J;Achtman M;Bentley SD;Nübel U
通讯作者：
Nübel U
影响因子：
4.9
作者：
Espedido, B. A.;Steen, J. A.;Jensen, S. O.
通讯作者：
Jensen, S. O.