Binary recombinase systems for high-resolution conditional mutagenesis.
Binary recombinase systems for high-resolution conditional mutagenesis.
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DOI：
10.1093/nar/gkt1361
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发表时间：
2014-04
影响因子：
14.9
通讯作者：
Pelczar P
中科院分区：
文献类型：
--
作者：
Hermann M;Stillhard P;Wildner H;Seruggia D;Kapp V;Sánchez-Iranzo H;Mercader N;Montoliu L;Zeilhofer HU;Pelczar P
使用组织特异性启动子表达的Cre重组酶进行条件诱变，有助于基因功能分析和细胞谱系追踪。在这里，我们描述了两种新的双启动子驱动的条件诱变系统，旨在提高重组的准确性和最佳效率。Co-Driver采用Dre和re-应答Cre的重组酶级联，仅当两种重组酶以预定的时间序列表达时才处理loxp -侧翼等位基因。这种独特的特性使Co-Driver成为旨在揭示细胞前体和成熟细胞类型之间关系的序列谱系追踪研究的理想选择。Co-InCre设计用于高效的交叉条件转基因。它依靠高度活跃的反式剪接蛋白和同时具有转录活性的启动子，从两个不活跃的前体片段中重建Cre重组酶。通过生成原生Cre， Co-InCre的重组率超过了本研究中评估的所有其他二元SSR系统。Co-Driver和Co-InCre都极大地扩展了现有的基因响应等位基因的效用。
Conditional mutagenesis using Cre recombinase expressed from tissue specific promoters facilitates analyses of gene function and cell lineage tracing. Here, we describe two novel dual-promoter-driven conditional mutagenesis systems designed for greater accuracy and optimal efficiency of recombination. Co-Driver employs a recombinase cascade of Dre and Dre-respondent Cre, which processes loxP-flanked alleles only when both recombinases are expressed in a predetermined temporal sequence. This unique property makes Co-Driver ideal for sequential lineage tracing studies aimed at unraveling the relationships between cellular precursors and mature cell types. Co-InCre was designed for highly efficient intersectional conditional transgenesis. It relies on highly active trans-splicing inteins and promoters with simultaneous transcriptional activity to reconstitute Cre recombinase from two inactive precursor fragments. By generating native Cre, Co-InCre attains recombination rates that exceed all other binary SSR systems evaluated in this study. Both Co-Driver and Co-InCre significantly extend the utility of existing Cre-responsive alleles.
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影响因子：
3.7
作者：
Engler, Carola;Kandzia, Romy;Marillonnet, Sylvestre
通讯作者：
Marillonnet, Sylvestre
裂解2：通过分裂蛋白片段互补介导的DNA重组的时间控制。
影响因子：
3.7
作者：
Hirrlinger J;Requardt RP;Winkler U;Wilhelm F;Schulze C;Hirrlinger PG
通讯作者：
Hirrlinger PG
DOI：
10.1006/bbrc.1997.7124
发表时间：
1997-08-28
影响因子：
3.1
作者：
Feil, R;Wagner, J;Chambon, P
通讯作者：
Chambon, P
DOI：
10.1126/science.1198704
发表时间：
2011-05-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bendall SC;Simonds EF;Qiu P;Amir el-AD;Krutzik PO;Finck R;Bruggner RV;Melamed R;Trejo A;Ornatsky OI;Balderas RS;Plevritis SK;Sachs K;Pe'er D;Tanner SD;Nolan GP
通讯作者：
Nolan GP
影响因子：
13.9
作者：
Kriegstein A;Alvarez-Buylla A
通讯作者：
Alvarez-Buylla A