Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients.
Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients.
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DOI：
10.1016/j.celrep.2021.108915
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发表时间：
2021-03-30
期刊：
影响因子：
8.8
通讯作者：
Tao SC
中科院分区：
文献类型：
--
作者：
Li Y;Ma ML;Lei Q;Wang F;Hong W;Lai DY;Hou H;Xu ZW;Zhang B;Chen H;Yu C;Xue JB;Zheng YX;Wang XN;Jiang HW;Zhang HN;Qi H;Guo SJ;Zhang Y;Lin X;Yao Z;Wu J;Sheng H;Zhang Y;Wei H;Sun Z;Fan X;Tao SC
为了全面解读SARS-CoV-2刺突蛋白的免疫原性，有必要系统地评估哪些部分具有高免疫原性。我们利用肽芯片分析了1051例2019冠状病毒病（COVID-19）患者的血清免疫球蛋白G （IgG）反应，生成了Spike蛋白的线性表位图。我们发现了两个富含线性表位的区域，即c -末端结构域（CTD）和靠近S2 '切割位点和融合肽的区域。出乎意料的是，我们发现受体结合域（RBD）缺乏线性表位。我们发现应答肽的数量在患者中是高度可变的，并且与疾病严重程度相关。一些多肽与严重程度和临床结果中度相关。通过免疫小鼠，我们获得了线性表位特异性抗体；然而，没有观察到这些抗体对真实病毒的显著中和活性。这将有助于我们了解sars - cov -2特异性体液反应，并可能有助于改进疫苗。Li等人基于大量的COVID-19患者队列构建了SARS-CoV-2刺突蛋白的B细胞线性表位景观。表位反应与疾病严重程度和预后有关，但主要引起非中和抗体。
To fully decipher the immunogenicity of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein, it is essential to assess which part is highly immunogenic in a systematic way. We generate a linear epitope landscape of the Spike protein by analyzing the serum immunoglobulin G (IgG) response of 1,051 coronavirus disease 2019 (COVID-19) patients with a peptide microarray. We reveal two regions rich in linear epitopes, i.e., C-terminal domain (CTD) and a region close to the S2′ cleavage site and fusion peptide. Unexpectedly, we find that the receptor binding domain (RBD) lacks linear epitope. We reveal that the number of responsive peptides is highly variable among patients and correlates with disease severity. Some peptides are moderately associated with severity and clinical outcome. By immunizing mice, we obtain linear-epitope-specific antibodies; however, no significant neutralizing activity against the authentic virus is observed for these antibodies. This landscape will facilitate our understanding of SARS-CoV-2-specific humoral responses and might be useful for vaccine refinement. Li et al. construct a B cell linear epitope landscape of SARS-CoV-2 Spike protein, based on a large cohort of COVID-19 patients. The epitope responses were related to disease severity and outcome but mainly elicit non-neutralizing antibodies.
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影响因子：
64.8
作者：
Barnes CO;Jette CA;Abernathy ME;Dam KA;Esswein SR;Gristick HB;Malyutin AG;Sharaf NG;Huey-Tubman KE;Lee YE;Robbiani DF;Nussenzweig MC;West AP Jr;Bjorkman PJ
通讯作者：
Bjorkman PJ
影响因子：
4.1
作者：
Herrera NG;Morano NC;Celikgil A;Georgiev GI;Malonis RJ;Lee JH;Tong K;Vergnolle O;Massimi AB;Yen LY;Noble AJ;Kopylov M;Bonanno JB;Garrett-Thomson SC;Hayes DB;Bortz RH 3rd;Wirchnianski AS;Florez C;Laudermilch E;Haslwanter D;Fels JM;Dieterle ME;Jangra RK;Barnhill J;Mengotto A;Kimmel D;Daily JP;Pirofski LA;Chandran K;Brenowitz M;Garforth SJ;Eng ET;Lai JR;Almo SC
通讯作者：
Almo SC
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
DOI：
10.1056/nejmoa2035389
发表时间：
2021-02-04
期刊：
The New England journal of medicine
影响因子：
--
作者：
Baden LR;El Sahly HM;Essink B;Kotloff K;Frey S;Novak R;Diemert D;Spector SA;Rouphael N;Creech CB;McGettigan J;Khetan S;Segall N;Solis J;Brosz A;Fierro C;Schwartz H;Neuzil K;Corey L;Gilbert P;Janes H;Follmann D;Marovich M;Mascola J;Polakowski L;Ledgerwood J;Graham BS;Bennett H;Pajon R;Knightly C;Leav B;Deng W;Zhou H;Han S;Ivarsson M;Miller J;Zaks T;COVE Study Group
通讯作者：
COVE Study Group
影响因子：
16.6
作者：
Custódio TF;Das H;Sheward DJ;Hanke L;Pazicky S;Pieprzyk J;Sorgenfrei M;Schroer MA;Gruzinov AY;Jeffries CM;Graewert MA;Svergun DI;Dobrev N;Remans K;Seeger MA;McInerney GM;Murrell B;Hällberg BM;Löw C
通讯作者：
Löw C