Recipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells.
Recipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells.
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受体骨髓同化不同胎儿造血干细胞的骨髓/淋巴重建
DOI：
10.18632/oncotarget.22479
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发表时间：
2017-12-12
期刊：
影响因子：
--
通讯作者：
Hong DL
中科院分区：
文献类型：
--
作者：
Guo XL;Chu L;Ke F;Mu LL;Li Z;Cai JJ;Li HF;Hong DL
胎肝是造血干细胞和祖细胞的重要来源。然而，在不同发育阶段收集的FL-HSPC在受体骨髓（BM）中是否相似或不同地重建仍不确定。我们在同种小鼠移植模型中研究了这个问题。我们首先分析了从受精后12.5天（dpf）到18.5 dpf的FL谱系组成。髓样和淋巴样细胞的绝对数量和比例是动态的。最大差异在12.5和16.5 dpf之间。然后将这两个时间点的FL-HSPC（Lin− CD 150 + CD 48 −）分别移植到受体中。在移植后4周或6周以及之后，谱系重建的差异是不可检测的，这表明BM环境同化了移植的细胞。对输入和输出HSPCs的谱系调控基因的分析表明，输入HSPCs的谱系调控基因的表达水平有很大差异，而移植HSPCs的谱系调控基因的表达水平基本相同。因此，受体BM微环境可以决定FL-HSPCs的发育谱系趋势。
The fetal liver (FL) is a source of hematopoietic stem and progenitor cells (HSPCs) for transplantation. However, whether FL-HSPCs collected at distinct developmental stages reconstitute similarly or differently in the recipient bone marrow (BM) remains undetermined. We examined this problem in a congeneic mouse transplantation model. We first analyzed the lineage components of FL from 12.5 days post-fertilization (dpf) to 18.5 dpf. The myeloid and lymphoid cells were dynamic in absolute number and ratio. The largest difference was between 12.5 and 16.5 dpf. The FL-HSPCs (Lin−CD150+CD48−) at these two time points were then respectively transplanted into the recipients. The difference in lineage reconstitution was undetectable at week 4 or 6 post-transplantation and afterward, indicating that the BM environment assimilated the transplanted cells. Profiling lineage-regulation genes of input and output HSPCs showed that the expression levels were much different in the former and almost the same in the engrafted HSPCs. Therefore, the recipient BM microenvironment could determine the developmental lineage-trends of FL-HSPCs.
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影响因子：
64.5
作者：
Tamplin OJ;Durand EM;Carr LA;Childs SJ;Hagedorn EJ;Li P;Yzaguirre AD;Speck NA;Zon LI
通讯作者：
Zon LI
DOI：
10.1126/science.aad0084
发表时间：
2016-01-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Khan JA;Mendelson A;Kunisaki Y;Birbrair A;Kou Y;Arnal-Estapé A;Pinho S;Ciero P;Nakahara F;Ma'ayan A;Bergman A;Merad M;Frenette PS
通讯作者：
Frenette PS
影响因子：
64.5
作者：
Kiel, MJ;Yilmaz, ÖH;Morrison, SJ
通讯作者：
Morrison, SJ
影响因子：
23.9
作者：
Dykstra, Brad;Kent, David;Eaves, Connie
通讯作者：
Eaves, Connie
影响因子：
4.8
作者：
Golfier, F;Bárcena, A;Muench, MO
通讯作者：
Muench, MO