Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes.
Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes.
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负压伤口治疗：通过微血管周细胞调节血流灌注和微血管成熟
DOI：
10.3892/ijmm.2017.3131
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发表时间：
2017-11
影响因子：
5.4
通讯作者：
Yu A
中科院分区：
文献类型：
--
作者：
Ma Z;Li Z;Shou K;Jian C;Li P;Niu Y;Qi B;Yu A
负压伤口治疗（NPWT）已被证明可通过促进血管生成加速伤口愈合。然而，NPWT后血流灌注是否受微血管成熟和周细胞调节，以及周细胞与IV型胶原蛋白之间的确切相关性仍不清楚。本研究的目的是研究NPWT后血流灌注与微血管成熟和周细胞之间的相关性，并进一步探索潜在的分子机制。我们还旨在研究周细胞和IV型胶原之间的关系。为此，我们建立了糖尿病大鼠伤口模型，并使用激光多普勒血流灌注成像仪检测微血管血流灌注。采用免疫组化、免疫荧光、RT-qPCR和western blot方法检测血管生成素-1、酪氨酸激酶受体-2（Tie-2）酪氨酸磷酸化、α-平滑肌肌动蛋白（α-SMA）和IV型胶原的表达水平。结果显示，在伤口愈合后期（7-10天），NPWT促进了血管生成素-1、Tie-2、α-SMA和IV型胶原的过度表达，并显著增加了NPWT组的血流灌注和微血管成熟。我们的结果表明，NPWT可以优先促进血管成熟，增加周细胞数量，从而调节血流灌注。另一方面，周细胞和IV型胶原蛋白相互作用，促进微血管成熟。
Negative pressure wound therapy (NPWT) has been demonstrated to accelerate wound healing by promoting angiogenesis. However, whether blood flow perfusion is regulated by microvessel maturation and pericytes following NPWT remains unclear, as well as the exact association between pericytes and collagen type IV. The aim of this study was to investigate the relevant association between blood flow perfusion and microvessel maturation and pericytes following NPWT, and to further explore the underlying molecular mechanisms. We also aimed to investigate the association between pericytes and collagen type IV. For this purpose, we created a rat model of diabetic wounds and microvascular blood flow perfusion was detected using a laser Doppler blood perfusion imager. The expression levels of angiogenin-1, tyrosine phosphorylation of tyrosine kinase receptor-2 (Tie-2), α-smooth muscle actin (α-SMA) and collagen type IV were detected and analyzed through immunohistochemistry, immunofluorescence, RT-qPCR and western blot analysis. The results revealed that NPWT promoted the overexpression of angiogenin-1, Tie-2, α-SMA and collagen type IV, and significantly increased blood flow perfusion coupled with microvessel maturation in the NPWT group at the later stages (7–10 days) of wound healing. Our results suggested that NPWT can preferentially enhance vessel maturation and increase the number of pericytes, thus regulating blood flow perfusion. On the other hand, pericytes and collagen type IV had a mutual interaction, promoting microvessel maturation.
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DOI：
10.1161/01.atv.0000256458.82320.be
发表时间：
2007-03-01
影响因子：
8.7
作者：
Kim, Kyung Eun;Cho, Chung-Hyun;Koh, Gou Young
通讯作者：
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DOI：
10.1038/jcbfm.2011.153
发表时间：
2012-02
期刊：
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影响因子：
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Østergaard L
影响因子：
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作者：
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通讯作者：
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影响因子：
64.8
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影响因子：
4.8
作者：
Hasenstein, Jason R.;Kasmerchak, Kelsey;Kozak, Kevin R.
通讯作者：
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