miR-218-5p in endometrial microenvironment prevents the migration of ectopic endometrial stromal cells by inhibiting LASP1.
miR-218-5p in endometrial microenvironment prevents the migration of ectopic endometrial stromal cells by inhibiting LASP1.
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DOI：
10.1186/s12958-022-00928-z
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发表时间：
2022-04-04
期刊：
影响因子：
--
通讯作者：
Huang O
中科院分区：
文献类型：
--
作者：
Zhang Z;Wang Y;Zeng L;Yu K;Wang Y;Luo Y;Liu F;Yang B;Zou Y;Wang L;Huang O
我们前期的双向电泳实验显示，LASP 1在子宫内膜异位症患者子宫内膜中的表达明显高于对照组子宫内膜。然而，LASP 1在子宫内膜异位症/子宫腺肌病中调节的分子机制尚不清楚。在此，进行qPCR以分析LASP 1和miR-218- 5 p在子宫内膜异位症（Ems）细胞和对照细胞之间的表达水平。采用荧光原位杂交技术检测miR-218- 5 p在异位内膜和正常子宫内膜中的表达水平。转染miR-218- 5 p模拟物或抑制剂后，通过transwell实验观察miR-218- 5 p对子宫内膜间质细胞（ESCs）迁移的影响。EdU法检测细胞增殖率;使用双荧光素酶报告基因测定来验证hsa-miR-218- 5 p与LASP 1的3 'UTR的结合。采用Western blot和免疫荧光法检测LASP 1和EMT标志物在子宫内膜组织中的表达情况。miR-218- 5 p主要由血管分泌，在子宫内膜周围的肌肉层中表达，其通过结合正常ESC中LASP 1的3 'UTR区域来抑制LASP 1的表达水平。miR-218- 5 p的过表达阻碍了上皮细胞向间充质细胞转化（EMT），并阻止了ESCs的迁移和Ems中波形蛋白的表达。我们的研究结果显示，子宫内膜微环境中的miR-218- 5 p通过抑制LASP 1阻止异位子宫内膜间质细胞的迁移。在线版本包含补充材料，可通过10.1186/s12958-022-00928-z获得。
Our previous two-dimensional electrophoresis experiment showed that the expression of LASP1 in patients with endometriosis was significantly higher than that of control endometrium. However, the molecular mechanism by which LASP1 is regulated in endometriosis/adenomyosis is unknown. Herein, qPCR was performed to analyze the expression levels of LASP1 and miR-218-5p between endometriosis (Ems) cells and control cells. Fluorescence in situ hybridization was carried out to measure the expression level of miR-218-5p in ectopic endometrium versus normal endometrium. After miR-218-5p mimic or inhibitor were transfected, the transwell experiment was carried out to see the effect of miR-218-5p on the migration of endometrial stromal cells (ESCs). EdU was used to measure cell proliferation rate. Dual-luciferase reporter assay was used to verify the binding of hsa-miR-218-5p to the 3’UTR of LASP1. Western blot and immunofluorescence analysis were carried out to identify the protein expression pattern of LASP1 and EMT markers in endometrial tissue. The miR-218-5p is mainly secreted from blood vessels and expressed in the muscle layer around the endometrium, which inhibits the expression level of LASP1 by binding the 3’UTR region of LASP1 in normal ESCs. Overexpression of miR-218-5p impedes the epithelial-to-mesenchymal transition (EMT) and prevents the migration of ESCs and the expression of Vimentin in Ems. Our findings revealed that miR-218-5p in endometrial microenvironment prevents the migration of ectopic endometrial stromal cells by inhibiting LASP1. The online version contains supplementary material available at 10.1186/s12958-022-00928-z.
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DOI：
10.1186/1756-9966-31-58
发表时间：
2012-06-19
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Wang C;Zheng X;Shen C;Shi Y
通讯作者：
Shi Y
影响因子：
5.2
作者：
Yamamoto N;Kinoshita T;Nohata N;Itesako T;Yoshino H;Enokida H;Nakagawa M;Shozu M;Seki N
通讯作者：
Seki N
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影响因子：
--
作者：
Jin Y;Xiong A;Zhang Z;Li S;Huang H;Yu TT;Cao X;Cheng SY
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影响因子：
4
作者：
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通讯作者：
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影响因子：
25.7
作者：
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