Ontogenesis of intestine morphology and intestinal disaccharidases in chickens (Gallus gallus) fed contrasting purified diets

Ontogenesis of intestine morphology and intestinal disaccharidases in chickens (Gallus gallus) fed contrasting purified diets
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饲喂对比纯化饲料的鸡(原鸡)肠道形态和肠道二糖酶的个体发生

DOI:
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发表时间:
1993
期刊:
Journal of Comparative Physiology □ B
影响因子:
--
通讯作者:
D. Phillips
D. Phillips
中科院分区:
--
文献类型:
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作者:
A. Biviano;C. Martinez del Rio;D. Phillips

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用含碳水化合物(52.5%)或不含碳水化合物的日粮饲喂康尼什×洛克杂交鸡两组。在孵化后36天,每组中的一些雏鸟被转移到相反的饲料中。与饲喂无碳水化合物饲料的鸡相比,饲喂含碳水化合物饲料的鸡生长更快,获得的渐近质量更高。喂食无碳水化合物饲料的鸡比喂含碳水化合物饲料的同等质量的鸡有更长的肠道和更大的肠道面积。在两组中,蔗糖酶和麦芽糖酶活性(以肠道面积或质量为标准)从第1天到大约第17天都有所增加。第17天后,饲喂含碳水化合物日粮的鸡单位肠道面积的蔗糖酶和麦芽糖酶活性分别是饲喂无碳水化合物日粮的鸡的1.8倍和1.9倍。用协方差分析估计蔗糖酶和蔗糖非依赖麦芽糖酶对麦芽糖酶活性的贡献,并估计日粮对蔗糖非依赖麦芽糖酶的影响。蔗糖酶对碳水化合物饲喂鸡麦芽糖酶活性的贡献率为80%,无糖饲喂鸡麦芽糖酶活性的贡献率为75%。在8天内,饲喂碳水化合物的鸡从无碳水化合物饲料转变为碳水化合物饲料,在大肠形态、肠道蔗糖酶和麦芽糖酶水平上趋于一致。相反,从碳水化合物饲料转变为无碳水化合物饲料的鸡,肠道长度没有明显变化,8天后蔗糖酶和麦芽糖酶的水平也没有降低。将整合麦芽糖酶肠道活动与已发表的葡萄糖摄取数据进行比较,发现麦芽糖的水解量与葡萄糖摄取量的比率似乎约为7,在个体发育过程中保持相对不变。由于对体内水解和摄取之间的相互作用知之甚少,因此很难确定这个相对较高的比率是否代表过多的水解力,或者是否需要它来提供最大限度摄取的高腔葡萄糖浓度。
Two groups of growing posthatching Cornish x Rock cross chickens were fed with either a carbohydrate-containing (52.5%) or a carbohydrate-free diet. At 36 days after hatching some of the chicks in each group were shifted to the opposite diet. Chickens fed on a carbohydrate-containing diet grew faster and achieved higher asymptotic masses than chickens fed on a carbohydrate-free diet. Chickens fed on a carbohydrate-free diet had longer intestines and larger intestinal areas than chickens of the same mass fed on a carbohydrate-containing diet. In both groups sucrase and maltase activity (standardized by either intestinal area or mass) increased from day 1 to approximately day 17. After day 17, chickens fed on a carbohydrate-containing diet exhibited 1.8 and 1.9 times higher sucrase and maltase activities per unit intestinal area, respectively, than chickens fed on a carbohydrate-free diet. Analysis of covariance was used to estimate the contribution of sucrase and the sucrase-independent maltases to maltase activity, and to estimate the effect of diet on the sucrase-independent maltases. Sucrase contributed 80% and 75% of the maltase activity in carbohydrate and carbohydrate-free fed chickens, respectively. Chickens shifted from a carbohydrate-free to a carbohydrate diet converged in gross intestinal morphology and intestinal sucrase and maltase levels with carbohydrate-fed chickens within 8 days. Chickens shifted from carbohydrate to carbohydrate-free diets, in contrast, did not show appreciable changes in intestinal length and after 8 days had not reduced levels of sucrase and maltase to those of chickens fed on the carbohydrate-free diet. A comparison of integrated maltase intestinal activity with published data on glucose uptake showed that the ratio of maltose hydrolysis to glucose uptake seemed to be about 7 and to remain relatively invariant during ontogeny. Because so little is known about the interaction between hydrolysis and uptake in vivo, it is difficult to determine if this relatively high ratio represents excess hydrolytic capacity or if it is needed to provide high lumenal glucose concentrations that maximize uptake.
DOI: 10.1073/pnas.80.24.7674
发表时间: 1983-12
影响因子: 11.1
作者:
W. Karasov;R. S. Pond;D. Solberg;J. Diamond
通讯作者: W. Karasov;R. S. Pond;D. Solberg;J. Diamond
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DOI: 10.1152/ajpgi.1989.256.1.g158
发表时间: 1989
期刊: The American journal of physiology
影响因子: --
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DOI: 10.1126/science.2916126
发表时间: 1989
期刊: Science (New York, N.Y.)
影响因子: --
作者:
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发表时间: 1990
期刊: The American journal of physiology
影响因子: --
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发表时间: 1980-12
期刊: The American journal of physiology
影响因子: --
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