Dendrobium mixture regulates hepatic gluconeogenesis in diabetic rats via the phosphoinositide-3-kinase/protein kinase B signaling pathway.
Dendrobium mixture regulates hepatic gluconeogenesis in diabetic rats via the phosphoinositide-3-kinase/protein kinase B signaling pathway.
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树突混合物通过磷酸肌醇-3-激酶/蛋白激酶B信号传导途径调节糖尿病大鼠中肝糖异生。
DOI：
10.3892/etm.2018.6194
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发表时间：
2018-07
影响因子：
2.7
通讯作者：
Wei M
中科院分区：
文献类型：
--
作者：
Lin X;Shi H;Cui Y;Wang X;Zhang J;Yu W;Wei M
本研究旨在探讨石杉合剂(DMix)对糖尿病大鼠肝脏胰岛素信号通路相关因子基因和蛋白表达的影响。并对DMix抑制糖异生的分子机制进行了探讨。本研究选用雌性Wistar大鼠47只。其中11只随机选为健康对照组，其余36只大鼠采用高脂高糖饮食6周后两次腹腔注射链脲佐菌素的方法建立糖尿病模型。对36只大鼠进行糖尿病筛查，然后随机分为3组：模型组、二甲双胍组和DMix组。治疗12周后检测空腹血糖(FBG)、糖化血清蛋白(GSP)、血清胰岛素、血脂[总胆固醇(Tch)、甘油三酯(TG)]、丙氨酸氨基转移酶(ALT)、天冬氨酸转氨酶(AST)。苏木精-伊红染色观察肝组织的组织形态变化。用逆转录-定量聚合酶链式反应检测肝脏胰岛素受体(InsR)、叉头盒蛋白O1(FoxO1)、磷酸烯醇式丙酮酸羧激酶(PEPCK)和葡萄糖6-磷酸酶(G6Pase)的mRNA表达，用免疫印迹法检测肝脏InsR、磷脂酰肌醇-3-激酶(PI3K)、磷酸化(P)-PI3K、蛋白激酶B(Akt)、p-Akt、FoxO1、PEPCK和G6Pase的蛋白表达。DMix治疗组的空腹血糖、GSP、InsR、Tch、TG、ALT、AST水平明显低于模型组(P<；0.05)。此外，DMix治疗可显著改善肝脏组织病理学改变，显著提高InsR、PI3K和Akt的基因和蛋白表达(P<；0.05)。DMix还显著降低了FoxO1、PEPCK和G6Pase的基因和蛋白表达(P<；0.05)。DMix通过调节PI3K/Akt信号通路相关分子的基因和蛋白表达，有效降低糖尿病大鼠的空腹血糖和血脂，显著改善糖尿病大鼠的肝功能和胰岛素抵抗。
The present study aimed to evaluate the impact of dendrobium mixture (DMix) on the gene and protein expression of insulin signaling pathway-associated factors in the livers of diabetic rats. The molecular mechanisms by which DMix inhibits gluconeogenesis were also investigated. A total of 47 female Wistar rats were used in the present study. Of these, 11 rats were randomly selected as healthy controls and diabetes was induced in the remaining 36 rats by administering a high-fat and high-sugar diet for 6 weeks, followed by two intraperitoneal injections of streptozotocin. The 36 rats were screened for diabetes and then randomly divided into three groups: Model, metformin and DMix groups. Following 12 weeks of treatment, the fasting blood glucose (FBG), glycosylated serum protein (GSP), serum insulin, blood lipids [total cholesterol (Tch) and triglycerides (TG)], alanine transaminase (ALT) and aspartate transaminase (AST) were assessed. In addition, hematoxylin and eosin staining was used for histomorphological examination of the liver tissues. The mRNA expression of insulin receptor (InsR), forkhead box protein O1 (FoxO1), phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase) in the liver was measured with reverse transcription-quantitative polymerase chain reaction and the protein expression of InsR, phosphoinositide-3-kinase (PI3K), phosphorylated (p)-PI3K, protein kinase B (Akt), p-Akt, FoxO1, PEPCK and G6Pase in the liver was measured by western blot analysis. The FBG, GSP, InsR, Tch, TG, ALT and AST levels were significantly lower in the DMix-treated group compared with the model group (P<0.05). In addition, DMix treatment notably improved liver histopathology and significantly increased the gene and protein expression of InsR, PI3K and Akt (P<0.05). DMix treatment also significantly reduced the gene and protein expression of FoxO1, PEPCK and G6Pase (P<0.05). DMix effectively reduced FBG and blood lipids and significantly improved liver function and insulin resistance in diabetic rats, possibly by regulating the gene and protein expression of molecules associated with the PI3K/Akt signaling pathway.
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影响因子：
7.7
作者：
Houde VP;Brûlé S;Festuccia WT;Blanchard PG;Bellmann K;Deshaies Y;Marette A
通讯作者：
Marette A
影响因子：
7.7
作者：
Nolan, Christopher J.;Ruderman, Neil B.;Prentki, Marc
通讯作者：
Prentki, Marc
通过结构优化鉴定出一种有前景的 PI3K 抑制剂用于治疗多发性骨髓瘤
影响因子：
28.5
作者：
Han K;Xu X;Chen G;Zeng Y;Zhu J;Du X;Zhang Z;Cao B;Liu Z;Mao X
通讯作者：
Mao X
影响因子：
4.2
作者：
Gross, Danielle N.;Wan, Min;Birnbaum, Morris J.
通讯作者：
Birnbaum, Morris J.
影响因子：
158.5
作者：
STUMVOLL, M;NURJHAN, N;GERICH, JE
通讯作者：
GERICH, JE