Proteomic analysis of human immunodeficiency virus and periodontitis
Proteomic analysis of human immunodeficiency virus and periodontitis
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人类免疫缺陷病毒与牙周炎的蛋白质组学分析
DOI：
10.1080/14789450.2020.1879648
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发表时间：
2020
影响因子：
3.4
通讯作者：
Nakayama Tomohiro
中科院分区：
文献类型：
--
作者：
Tsuchida Sachio;Nakayama Tomohiro
牙周病是一种慢性口腔感染，由牙科生物膜堆积对牙龈上组织长期持续的刺激引起，可导致结缔组织、牙骨质、牙周膜和牙槽骨的破坏[1-3]。几项研究的结果表明，牙周炎与系统性和主要生活方式相关的疾病有关，因为牙周炎被认为是由某些类型的细菌、不良饮食习惯和吸烟引发的[4-7]。Ahmad等人。报告称，阅读量最高的100种出版物涉及的主题大多涉及牙周病与心血管疾病(8598条)和糖尿病(7660条)的关系，其次是牙周炎的全身并发症(混合表现；3918条引文)，与妊娠相关的表现(10,909条引文)，风湿表现(1955条引文)，肺部表现(399条引文)，脑血管疾病(478条)和癌症(169条)[8]。然而，牙周炎的发病机制尚不清楚。细菌病原体与牙周病的发生和发展有关[9，10]。在讨论牙周缝隙的微生物组成之前，应考虑牙科微生物生物膜的一些更一般的方面，包括牙科生物膜的发育、异质性、微生物演替、组成、结构和形成机制。牙科生物膜是与牙齿表面密切相关的异质、致密和非钙化的细菌团的集合，它们牢固地附着在表面上通常被认为是为了防止它们随着唾液流动而移位。龈下生物膜是一种非粘附性生物膜，由大量可移动的生物组成。然而，全身性疾病与牙周病相关的详细机制仍不清楚。基于MS的方法可以量化生物液中的酶水平，但它们缺乏测量其酶活性的能力[11]。疾病状态的机制尚不清楚，尽管据报道牙周病的发病是由细菌牙周感染引发的。病毒可能通过改变免疫防御或诱导破坏性宿主反应，或通过直接溶解牙周组织而参与牙周炎的发病[12，13]。蛋白质组定义了生物体、组织或细胞能够表达的一整套蛋白质，由于它可以直接导致靶向治疗的发展和疾病标志物的识别，因此引起了人们的极大关注。近年来，蛋白质组学技术被用于鉴定牙周炎的生物标志物，经过详尽的研究发现了几个有希望的候选者[14-17]。为了确定生物标志物的一致性，提取的蛋白质通过一维和二维凝胶电泳法分离，并用基质辅助激光解吸/电离飞行时间质谱仪(MALDI-TOF)测量它们的磁荷比。液-质联用是另一种用于蛋白质鉴定的工具，对于筛选与先天代谢错误、毒理学分析和确定药物疗效有关的蛋白质尤其不可或缺。此外，各种免疫分析经常被用来检测或测量类固醇和多肽激素的浓度。从历史上看，蛋白质检测方法一直是确定牙周病过程中调节的一些途径的基础：…
Periodontal disease is a chronic oral infection that results from prolonged and persistent irritation of the supragingival tissue by dental biofilm buildup and can result in the destruction of connective tissue, cementum, the periodontal ligament, and the alveolar bone [1–3]. The findings of several studies have indicated a relationship between periodontitis and systemic and major lifestyle-related diseases, inasmuch as periodontitis is considered to be triggered by certain types of bacteria, poor dietary habits, and smoking [4–7]. Ahmad et al. reported that the majority of topics covered by the top 100 most widely read publications concerned the associations of periodontal disease with cardiovascular diseases (8598 citations) and with diabetes mellitus (7660 citations), followed by the systemic complications of periodontitis (mixed manifestations; 3918 citations), pregnancyrelated manifestations (10,909 citations), rheumatic manifestations (1955 citations), pulmonary manifestations (399 citations), cerebrovascular diseases (478 citations), and cancer (169 citations)[8]. However, the mechanisms underlying periodontitis are unclear. Bacterial pathogens are associated with the onset and progression of periodontal disease [9, 10]. Consideration of some of the more general aspects of microbial dental biofilm, including the development, heterogeneity, microbial succession, composition, structure, and mechanisms of dental biofilm formation, should precede the discussion of the microbial composition of the gingival crevice (subgingival dental biofilm). Dental biofilm is a collection of heterogeneous, dense, and non-calcified bacterial masses intimately associated with the tooth surface, and their firm adherence to the surface is usually considered to prevent their dislodgement with the salivary flow. Subgingival biofilms are nonadherent and comprise a large number of motile organisms. However, the detailed mechanism by which systemic disease is related to periodontal disease remains unclear. MS-based approaches can quantify enzyme levels in biological fluids, but they lack the capacity to measure their enzymatic activity [11]. The mechanistic aspects underlying the disease state are unknown, although the onset of periodontal disease is reportedly triggered by bacterial periodontal infection. Viruses may participate in the pathogenesis of periodontitis by altering immunological defenses or inducing destructive host reactions, or through direct lytic effects on periodontal tissue [12, 13]. Proteome, which defines the entire set of proteins that can be expressed by an organism, tissue, or cell, has attracted considerable attention because it can directly lead to the development of targeted therapeutics and identification of disease markers. In recent years, proteomic technology has been used to identify periodontitis biomarkers, with the discovery of several promising candidates as a result of exhaustive studies [14–17]. To determine the identity of biomarkers, extracted proteins are separated by one-and two-dimensional gel electrophoresis and their massto-charge ratios are measured with matrix-assisted laser desorption/ionization–time-of-flight (MALDI–TOF) mass spectrometry (MS). Liquid chromatography (LC)–MS, another tool used for protein identification, is particularly indispensable for screening proteins involved in inborn metabolism errors, toxicological analyses, and determining the efficacy of drugs. In addition, various immunoassays are frequently used to detect or measure steroid and peptide hormone concentrations. Historically, protein detection approaches have served as the basis to define a number of pathways regulated during the course of periodontal disease …
病毒引起的中性粒细胞功能抑制及其在继发性微生物感染中的作用。
DOI：
10.1093/clinids/10.2.326
发表时间：
1988
期刊：
Reviews of infectious diseases
影响因子：
--
作者：
Abramson,JS;Mills,EL
通讯作者：
Mills,EL
影响因子：
3.4
作者：
Batschkus, Sarah;Cingoez, Goekhan;Lenz, Christof
通讯作者：
Lenz, Christof