Functional Study and Efficient Catalytic Element Mining of CYP76AHs in Salvia Plants.
Functional Study and Efficient Catalytic Element Mining of CYP76AHs in Salvia Plants.
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DOI：
10.3390/molecules28124711
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发表时间：
2023-06-12
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
鼠尾草属是一个大属，有数百种用于传统中药。丹参酮是鼠尾草属中发现的一类具有高度代表性的独特化合物，具有显著的生物活性。从16种鼠尾草属植物中鉴定出丹参酮类成分。CYP 76 AH亚家族（P450）由于其催化产生多羟基结构而对丹参酮的合成至关重要。本研究共获得420个CYP 76 AH基因，系统发育分析显示它们之间有明显的聚类关系。本研究克隆了10种鼠尾草属植物的15个CYP 76 AH基因，并从进化和催化效率的角度进行了研究。与SmCYP 76 AH 3相比，发现3个CYP 76 AH 3具有显著提高的催化效率，为丹参酮的生物合成提供了有效的催化元件。结构-功能关系研究揭示了几个可能与CYP 76 AHs功能相关的保守残基，为植物P450的定向进化研究提供了新的突变方向。
Salvia is a large genus with hundreds of species used in traditional Chinese medicine. Tanshinones are a highly representative class of exclusive compounds found in the Salvia genus that exhibit significant biological activity. Tanshinone components have been identified in 16 Salvia species. The CYP76AH subfamily (P450) is crucial for the synthesis of tanshinone due to its catalytic generation of polyhydroxy structures. In this study, a total of 420 CYP76AH genes were obtained, and phylogenetic analysis showed their clear clustering relationships. Fifteen CYP76AH genes from 10 Salvia species were cloned and studied from the perspectives of evolution and catalytic efficiency. Three CYP76AHs with significantly improved catalytic efficiency compared to SmCYP76AH3 were identified, providing efficient catalytic elements for the synthetic biological production of tanshinones. A structure–function relationship study revealed several conserved residues that might be related to the function of CYP76AHs and provided a new mutation direction for the study of the directed evolution of plant P450.
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影响因子：
5.6
作者：
Fang ZY;Zhang M;Liu JN;Zhao X;Zhang YQ;Fang L
通讯作者：
Fang L
影响因子：
6.4
作者：
Forman V;Bjerg-Jensen N;Dyekjær JD;Møller BL;Pateraki I
通讯作者：
Pateraki I
影响因子：
12.9
作者：
Kwon, Moonhyuk;Utomo, Joseph C.;Ro, Dae-Kyun
通讯作者：
Ro, Dae-Kyun
影响因子：
16.6
作者：
Ma Y;Cui G;Chen T;Ma X;Wang R;Jin B;Yang J;Kang L;Tang J;Lai C;Wang Y;Zhao Y;Shen Y;Zeng W;Peters RJ;Qi X;Guo J;Huang L
通讯作者：
Huang L
影响因子：
4.8
作者：
Urban, P;Mignotte, C;Pompon, D
通讯作者：
Pompon, D