The effect of alcohols on red blood cell mechanical properties and membrane fluidity depends on their molecular size.
The effect of alcohols on red blood cell mechanical properties and membrane fluidity depends on their molecular size.
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DOI：
10.1371/journal.pone.0076579
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Baskurt OK
中科院分区：
文献类型：
--
作者：
Sonmez M;Ince HY;Yalcin O;Ajdžanović V;Spasojević I;Meiselman HJ;Baskurt OK
许多研究表明，膜流动性在决定红细胞(RBC)变形性中的作用。本研究采用红细胞比色法和电子顺磁共振(EPR)技术研究了四种直链醇(甲醇、乙醇、丙醇和正丁醇)对红细胞膜流动性、变形性和稳定性的影响。所有醇对变形性都有双相效应，随着浓度的增加，变形性先增大后减小；反转的临界浓度与分子大小成反比。EPR结果显示近表面流动性呈双相变化(即先增加后减少)和脂核流动性降低，其效果顺序为丁醇和丙醇；乙醇和甲醇，且近表面流动性与变形性显著相关(r=0.697；p<；0.01)。酒精的存在增加了细胞承受100pa切应力所引起的红细胞变形能力的损害，在所有四种酒精浓度较高的情况下，观察到与对照组有显著差异。溶血程度取决于分子大小和浓度，而只有乙醇和丙醇才能观察到棘细胞形状的转变(即双凹盘状到锯齿状)。这些结果与在模型膜上获得的现有数据一致。他们证明了红细胞变形性和近表面膜流动性之间存在机械联系，醇改变红细胞机械行为的能力与链长有关，以及红细胞变形性和近表面膜流动性对酒精浓度增加的双相反应。
The role of membrane fluidity in determining red blood cell (RBC) deformability has been suggested by a number of studies. The present investigation evaluated alterations of RBC membrane fluidity, deformability and stability in the presence of four linear alcohols (methanol, ethanol, propanol and butanol) using ektacytometry and electron paramagnetic resonance (EPR) spectroscopy. All alcohols had a biphasic effect on deformability such that it increased then decreased with increasing concentration; the critical concentration for reversal was an inverse function of molecular size. EPR results showed biphasic changes of near-surface fluidity (i.e., increase then decrease) and a decreased fluidity of the lipid core; rank order of effectiveness was butanol > propanol > ethanol > methanol, with a significant correlation between near-surface fluidity and deformability (r = 0.697; p<0.01). The presence of alcohol enhanced the impairment of RBC deformability caused by subjecting cells to 100 Pa shear stress for 300 s, with significant differences from control being observed at higher concentrations of all four alcohols. The level of hemolysis was dependent on molecular size and concentration, whereas echinocytic shape transformation (i.e., biconcave disc to crenated morphology) was observed only for ethanol and propanol. These results are in accordance with available data obtained on model membranes. They document the presence of mechanical links between RBC deformability and near-surface membrane fluidity, chain length-dependence of the ability of alcohols to alter RBC mechanical behavior, and the biphasic response of RBC deformability and near-surface membrane fluidity to increasing alcohol concentrations.
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DOI：
10.3109/00365510903266069
发表时间：
2009-01-01
影响因子：
2.1
作者：
Baskurt, Oguz K.;Hardeman, Max R.;Meiselman, Herbert J.
通讯作者：
Meiselman, Herbert J.
影响因子：
3.3
作者：
Gurtovenko, Andrey A.;Anwar, Jamshed
通讯作者：
Anwar, Jamshed
影响因子：
2.9
作者：
BARRY, JA;GAWRISCH, K
通讯作者：
GAWRISCH, K
影响因子：
2.9
作者：
ALMEIDA, LM;VAZ, WLC;MADEIRA, VMC
通讯作者：
MADEIRA, VMC
影响因子：
2.4
作者：
Kaye, Martin D.;Schmalzl, Karin;Rheinstaedter, Maikel C.
通讯作者：
Rheinstaedter, Maikel C.