Genome wide analysis of the bovine mucin genes and their gastrointestinal transcription profile.
Genome wide analysis of the bovine mucin genes and their gastrointestinal transcription profile.
复制标题
DOI：
10.1186/1471-2164-12-140
复制
发表时间：
2011-03-07
期刊：
影响因子：
4.4
通讯作者：
Geldhof P
中科院分区：
文献类型：
--
作者：
Hoorens PR;Rinaldi M;Li RW;Goddeeris B;Claerebout E;Vercruysse J;Geldhof P
粘蛋白是涉及保护所有粘膜表面的大的糖蛋白。在人类和啮齿类动物中，粘蛋白基因家族已被充分描述，并且先前的研究已经调查了粘蛋白在胃肠道（GI）中的分布和功能。相反，关于多胃物种（如牛）中粘蛋白基因家族的数据很少。本研究的目的是通过基因组挖掘鉴定牛粘蛋白家族的所有成员，并随后研究这些粘蛋白在胃肠道中的转录模式。在牛基因组中鉴定了9种牛膜相关粘蛋白（MUC1、MUC3A、MUC4、MUC12、MUC13、MUC15、MUC16、MUC20和MUC21）和6种分泌型粘蛋白（MUC2、MUC5AC、MUC5B、MUC6、MUC7和MUC19）。对于MUC3B、MUC8和MUC17，未鉴定出同源物。一般来说，膜相关粘蛋白的结构域架构被发现是相似的人和牛之间，而凝胶形成粘蛋白的蛋白质结构似乎是不太保守。基因组组织的进一步分析表明，以前报道的牛颌下粘蛋白（BSM）可能是一个更大的基因编码MUC19的一部分。转录谱分析表明，分泌的粘蛋白从皱胃开始转录，而膜相关粘蛋白MUC1和MUC20在整个胃肠道中转录。与人类相反，MUC 5B转录本在小肠和大肠中都被发现，但在食道组织中不存在。这项研究提供了第一个特征的粘蛋白基因家族在牛和他们的转录调控在胃肠道。本文中提出的数据将允许进一步研究这些蛋白质在反刍动物胃肠道的生理和它们与病原体的相互作用。
Mucins are large glycoproteins implicated in protection of all mucosal surfaces. In humans and rodents, the mucin gene family has been well described and previous studies have investigated the distribution and function of mucins in the gastrointestinal (GI) tract. In contrast, little data is available on the mucin gene family in polygastric species, such as cattle. The aim of the current study was to identify all members of the bovine mucin family by genome mining and subsequently investigate the transcription pattern of these mucins in the GI tract. Nine bovine membrane-associated mucins (MUC1, MUC3A, MUC4, MUC12, MUC13, MUC15, MUC16, MUC20 and MUC21) and six secreted mucins (MUC2, MUC5AC, MUC5B, MUC6, MUC7 and MUC19) were identified in the bovine genome. No homologues could be identified for MUC3B, MUC8 and MUC17. In general, domain architecture of the membrane-associated mucins was found to be similar between humans and cattle, while the protein architecture of the gel-forming mucins appeared to be less conserved. Further analysis of the genomic organization indicated that the previously reported bovine submaxillary mucin (BSM) may be part of a larger gene encoding for MUC19. Analysis of the transcription profile showed that the secreted mucins were transcribed from the abomasum onwards, whereas the membrane associated mucins MUC1 and MUC20 were transcribed throughout the whole GI tract. In contrast to humans, MUC5B transcript was found in both the small and large intestine, but was absent in oesophageal tissue. This study provides the first characterization of the mucin gene family in cattle and their transcriptional regulation in the GI tract. The data presented in this paper will allow further studies of these proteins in the physiology of the GI tract in ruminants and their interactions with pathogens.
登录
查看更多内容
DOI：
10.1073/pnas.0705984104
发表时间：
2007-10-09
影响因子：
11.1
作者：
Lang, Tiange;Hansson, Gunnar C.;Samuelsson, Tore
通讯作者：
Samuelsson, Tore
影响因子：
4.8
作者：
Axelsson, MAB;Asker, N;Hansson, GC
通讯作者：
Hansson, GC
影响因子：
4.1
作者：
Jiang, WP;Woitach, JT;Bhavanandan, VP
通讯作者：
Bhavanandan, VP
影响因子：
4.8
作者：
Godl, K;Johansson, MEV;Hansson, GC
通讯作者：
Hansson, GC
DOI：
10.1006/bbrc.2002.6475
发表时间：
2002-03-01
影响因子：
3.1
作者：
Gum, JR;Crawley, SC;Kim, YS
通讯作者：
Kim, YS