Genetic Architecture and Genome-Wide Adaptive Signatures Underlying Stem Lenticel Traits in Populus tomentosa.
Genetic Architecture and Genome-Wide Adaptive Signatures Underlying Stem Lenticel Traits in Populus tomentosa.
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毛白杨茎皮孔性状的遗传结构和全基因组适应性特征
DOI：
10.3390/ijms22179249
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发表时间：
2021-08-26
影响因子：
5.6
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Li P;Zhou J;Wang D;Li L;Xiao L;Quan M;Lu W;Yao L;Fang Y;Lv C;Song F;Du Q;Zhang D
茎干细胞是木本植物的高度特化的组织，其已经进化为平衡茎水分保持和气体交换以适应当地环境。在这项研究中，我们应用全基因组关联研究和选择性扫描分析来表征303个无关的毛白杨种质的遗传结构和全基因组适应性特征，毛白杨在其自然分布的气候区域中具有显着的表型和遗传变异。总的来说，我们检测到108个显著的单核苷酸多态性，注释到88个候选基因，其中9个致病基因在气候区域之间显示出显着不同的选择签名。此外，PtoNAC083和PtoMYB46显示出显著的关联信号和非生物胁迫响应，因此我们在拟南芥中过表达这两个基因，发现所有三个过表达系中的干细胞数量都因PtoNAC083过表达而显著减少，但因PtoMYB46过表达而略有增加，这表明这两个基因都参与了细胞分裂和扩增过程中的细胞分裂和细胞增殖。这项研究的结果表明，成功应用的综合战略解剖复杂适应性状的遗传基础和景观遗传学，这将有助于分子设计的树木ideotypes，可能会适应未来的气候和环境变化。
The stem lenticel is a highly specialized tissue of woody plants that has evolved to balance stem water retention and gas exchange as an adaptation to local environments. In this study, we applied genome-wide association studies and selective sweeping analysis to characterize the genetic architecture and genome-wide adaptive signatures underlying stem lenticel traits among 303 unrelated accessions of P. tomentosa, which has significant phenotypic and genetic variations according to climate region across its natural distribution. In total, we detected 108 significant single-nucleotide polymorphisms, annotated to 88 candidate genes for lenticel, of which 9 causative genes showed significantly different selection signatures among climate regions. Furthermore, PtoNAC083 and PtoMYB46 showed significant association signals and abiotic stress response, so we overexpressed these two genes in Arabidopsis thaliana and found that the number of stem cells in all three overexpression lines was significantly reduced by PtoNAC083 overexpression but slightly increased by PtoMYB46 overexpression, suggesting that both genes are involved in cell division and expansion during lenticel formation. The findings of this study demonstrate the successful application of an integrated strategy for dissecting the genetic basis and landscape genetics of complex adaptive traits, which will facilitate the molecular design of tree ideotypes that may adapt to future climate and environmental changes.
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影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
4
作者：
Chamaillard, Sylvain;Fichot, Regis;Brignolas, Franck
通讯作者：
Brignolas, Franck
影响因子：
3.8
作者：
Collevatti, Rosane G.;Novaes, Evandro;Grattapaglia, Dario
通讯作者：
Grattapaglia, Dario
SSR标记揭示毛白杨遗传多样性和种群结构
影响因子：
3.1
作者：
Du, Qingzhang;Wang, Bowen;Li, Bailian
通讯作者：
Li, Bailian
影响因子：
3.3
作者：
Hansen, Thomas F.
通讯作者：
Hansen, Thomas F.