Activation of adipose tissue macrophages in obese mice does not require lymphocytes.
Activation of adipose tissue macrophages in obese mice does not require lymphocytes.
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DOI：
10.1002/oby.20159
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发表时间：
2013-07
期刊：
影响因子：
6.9
通讯作者：
Mittelman, Steven D.
中科院分区：
文献类型：
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作者：
Behan, James W.;Ehsanipour, Ehsan A.;Sheng, Xia;Pramanik, Rocky;Wang, Xingchao;Hsieh, Yao-Te;Kim, Yong-Mi;Mittelman, Steven D.
浸润脂肪组织并分泌促炎细胞因子的巨噬细胞可能是肥胖诱导的胰岛素抵抗的原因。然而，巨噬细胞为何迁移到脂肪组织并被激活仍然未知，尽管一些研究表明这可能受T淋巴细胞和B淋巴细胞的调节。在本研究中，我们测试T淋巴细胞、B淋巴细胞和自然杀伤（NK）细胞对于肥胖诱导的脂肪组织中巨噬细胞的激活是否是必需的。
NOD/SCID/IL2 - 受体γ链敲除（NSG）小鼠缺乏成熟的T淋巴细胞、B淋巴细胞和NK细胞，通过选择性减少产仔数并在断奶后给予高脂饮食使其肥胖。然后小鼠持续这种饮食10 - 11周。肥胖的NSG小鼠的脂肪组织比非肥胖小鼠有更多的活化巨噬细胞。这些巨噬细胞在脂肪细胞周围的“冠状结构”中被发现，并且表达更高水平的炎症细胞因子肿瘤坏死因子α（TNFα）。然而，肥胖并没有损害NSG小鼠的葡萄糖耐量。
这些研究表明，T淋巴细胞、B淋巴细胞和NK细胞对于肥胖小鼠脂肪组织巨噬细胞的激活不是必需的。T淋巴细胞、B淋巴细胞和/或NK细胞对于肥胖诱导的葡萄糖耐量受损的发展可能是必需的。
Macrophages which infiltrate adipose tissue and secrete pro-inflammatory cytokines may be responsible for obesity-induced insulin resistance. However, why macrophages migrate into adipose tissue and become activated remains unknown, though some studies suggest this may be regulated by T and B lymphocytes. In the present study, we test whether T and B lymphocytes and NK cells are necessary for the obesity-induced activation of macrophages in adipose tissue. NOD/SCID/IL2-receptor gamma-chain knockout (NSG) mice, which lack mature T and B lymphocytes and NK cells, were made obese by selectively reducing litters and weaning onto a high-fat diet. Mice were then maintained on the diet for 10-11 weeks. Adipose tissue from obese NSG mice had more activated macrophages than non-obese mice. These macrophages were found in “crown like structures” surrounding adipocytes, and expressed higher levels of the inflammatory cytokine TNFα. However, obesity did not impair glucose tolerance in the NSG mice. These studies demonstrate that T and B lymphocytes and NK cells are not necessary for adipose tissue macrophage activation in obese mice. T and B lymphocytes and/or NK cells may be necessary for the development of obesity-induced impaired glucose tolerance.
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影响因子：
4.9
作者：
O'Rourke, R. W.;Metcalf, M. D.;White, A. E.;Madala, A.;Winters, B. R.;Maizlin, I. I.;Jobe, B. A.;Roberts, C. T., Jr.;Slifka, M. K.;Marks, D. L.
通讯作者：
Marks, D. L.
影响因子：
7.7
作者：
Lumeng, Carey N.;DeYoung, Stephanie M.;Saltiel, Alan R.
通讯作者：
Saltiel, Alan R.
影响因子：
3.6
作者：
Vaughan, Tamisha;Li, Liwu
通讯作者：
Li, Liwu
影响因子：
1.4
作者：
DOBUSH, GR;ANKNEY, CD;KREMENTZ, DG
通讯作者：
KREMENTZ, DG
影响因子：
82.9
作者：
通讯作者：
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