Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum
Characterization and functional analysis of hsp21.8b: An orthologous small heat shock protein gene in Tribolium castaneum
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hsp21.8b 的表征和功能分析：赤拟谷盗中的直系同源小热休克蛋白基因
DOI：
10.1111/jen.12519
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发表时间：
2018-08
影响因子：
1.9
通讯作者：
Li B.
中科院分区：
文献类型：
--
作者：
Xie J.;Xiong W.;Hu X.;Gu S.;Zhang S.;Gao S.;Song X.;Bi J.;Li B.
小热休克蛋白（sHSPs）作为分子伴侣蛋白广泛存在于各种生物体中。比较分析表明，在昆虫进化过程中存在同源shsp。本研究鉴定出一种昆虫同源shsp hsp21.8b。实时荧光定量PCR结果显示，Tchsp21.8b在所有发育阶段均有表达，在胚胎早期表达最低，在其他阶段表达相对较高，尤其是卵晚期和蛹晚期。成虫时期，Tchsp21.8b在中枢神经系统中表达量最高，其次是鞘翅、表皮、卵巢和脂肪体。此外，该基因在高温胁迫（45°C）下上调了3.39倍，在低温胁迫（4°C）下上调了1.73 - 1.94倍，在紫外线（UV）照射4 - 6小时时上调了1.73 - 1.94倍。在3 d饥饿条件下，其表达量下调20.8% ~ 41.8%，呈“下降-上升-下降”趋势。干扰Tchsp21.8b的幼虫RNA导致40.6%的昆虫死亡率和66.0%的产卵量减少，只有21.0%的ds‐Tchsp21.8b卵能孵化成幼虫。这些结果表明，Tchsp21.8b作为一种同源hsps，不仅在castaneum的生长发育和繁殖中发挥重要作用，而且具有抵抗环境胁迫的能力，尽管与其他hsps相比，其响应相对较弱。本研究还揭示了同源shsp在木栗的发育和抗胁迫能力中的作用。这为揭示昆虫鸣叫的生理机制和提高防治不同害虫的能力提供了更多的科学依据。
Small heat shock proteins (sHSPs) act as molecular chaperones and are widely distributed in all kinds of organisms. Comparative analysis revealed that an orthologous shsp was present during insect evolution. Here, hsp21.8b, one insect orthologous shsp, had been identified in Tribolium castaneum. Quantitative real‐time PCR illustrated that Tchsp21.8b was expressed in all developmental stages, along with the lowest expression at early embryonic stage and relative high expression at other stages especially in late eggs and late pupae. In the adult period, Tchsp21.8b exhibited the highest expression level in central nervous system and followed in elytron, epidermis, ovary and fat body. Moreover, it was upregulated 3.39‐fold in response to enhanced heat stress (45°C) for 4 hr but not to cold stress (4°C) and was upregulated by 1.73‐ to 1.94‐fold under ultraviolet (UV) exposure during 4–6 hr. It was also downregulated by 20.8%–41.8% under starvation in 3 days and had a “down‐up‐down” trend under the pathogen stresses. Larval RNA interference of Tchsp21.8b caused 40.6% insects mortality and reduced the oviposition amount by 66.0% and only 21.0% of the ds‐Tchsp21.8b eggs could hatch into larvae. These results suggested that as an orthologous shsp, Tchsp21.8b not only plays important roles in the growth, development and fecundity of T. castaneum but with the competence to resist the environment stresses, although the response is relatively weak compared to other hsps. Results from this study also uncovered the functions of the orthologous shsp in the development and anti‐stresses ability of T. castanuem. It provided more scientific evidence for revealing the physiological mechanisms of shsps of the insects and enhanced the capabilities to control different pests.
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赤拟谷盗基于 ER 的 HSP90 功能和信号通路的比较 RNA 测序分析。
DOI：
10.1007/s12192-017-0821-x
发表时间：
2018
期刊：
Cell Stress Chaperones
影响因子：
--
作者：
Xiong Wenfeng;Zhai Mengfan;Yu Xiaojuan;Wei Luting;Mao Jinjuan;Liu Juanjuan;Xie Jia;Li Bin
通讯作者：
Li Bin
影响因子：
3.9
作者：
Franck, E;Madsen, O;de Jong, WW
通讯作者：
de Jong, WW
影响因子：
2.9
作者：
Contreras, Estefania;Benito-Jardon, Maria;Rausell, Carolina
通讯作者：
Rausell, Carolina
影响因子：
10.7
作者：
W. D. de Jong;J. Leunissen;C. Voorter
通讯作者：
W. D. de Jong;J. Leunissen;C. Voorter
影响因子：
3.8
作者：
Lu, Ming-Xing;Hua, Jin;Du, Yu-Zhou
通讯作者：
Du, Yu-Zhou