Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes.
Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes.
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基于结构/裂解的对嗜热嗜热菌 Argonaute 沉默复合物内凸起位点螺旋扰动的见解
DOI：
10.1093/nar/gkx547
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发表时间：
2017-09-06
影响因子：
14.9
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Sheng G;Gogakos T;Wang J;Zhao H;Serganov A;Juranek S;Tuschl T;Patel DJ;Wang Y
Abstract We have undertaken a systematic structural study of Thermus thermophilus Argonaute (TtAgo) ternary complexes containing single-base bulges positioned either within the seed segment of the guide or target strands and at the cleavage site. Our studies establish that single-base bulges 7T8, 5A6 and 4A5 on the guide strand are stacked-into the duplex, with conformational changes localized to the bulge site, thereby having minimal impact on the cleavage site. By contrast, single-base bulges 6’U7’ and 6’A7’ on the target strand are looped-out of the duplex, with the resulting conformational transitions shifting the cleavable phosphate by one step. We observe a stable alignment for the looped-out 6’N7’ bulge base, which stacks on the unpaired first base of the guide strand, with the looped-out alignment facilitated by weakened Watson–Crick and reversed non-canonical flanking pairs. These structural studies are complemented by cleavage assays that independently monitor the impact of bulges on TtAgo-mediated cleavage reaction.
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影响因子：
16
作者：
Olovnikov, Ivan;Chan, Ken;Sachidanandam, Ravi;Newman, Dianne K.;Aravin, Alexei A.
通讯作者：
Aravin, Alexei A.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
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作者：
Chi, Sung Wook;Hannon, Gregory J.;Darnell, Robert B.
通讯作者：
Darnell, Robert B.