Crystal structures of complexes between aminoglycosides and decoding A site oligonucleotides: role of the number of rings and positive charges in the specific binding leading to miscoding.
Crystal structures of complexes between aminoglycosides and decoding A site oligonucleotides: role of the number of rings and positive charges in the specific binding leading to miscoding.
复制标题
氨基糖苷与解码位点寡核苷酸之间的复合物的晶体结构：环数和正电荷在特定结合中导致错误编码的作用。
DOI：
10.1093/nar/gki862
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发表时间：
2005
影响因子：
14.9
通讯作者：
Westhof, E
中科院分区：
文献类型：
--
作者：
François, B;Russell, RJM;Murray, JB;Aboul-ela, F;Masquida, B;Vicens, Q;Westhof, E
氨基糖苷类抗生素（奈胺、庆大霉素C1A、卡那霉素A、核糖素、利维霉素A和新霉素B）与含有细菌核糖体解码A位点的寡核苷酸之间的六种配合物的晶体结构在2.2和3.0之间的分辨率被报道Å。虽然RNA与氨基糖苷之间的接触数在20到31之间变化，但在观察到的配合物中，氨基部分的I和II环之间保留了多达8个直接氢键。褶皱糖环I通过与G1491叠加插入A位点螺旋，与普遍保守的A1408的沃森-克里克位点形成两个h键的伪碱基对。这种中心相互作用有助于维持A1492和A1493的突出构象。所有这些与各种氨基糖苷络合的最小A位点RNA结构都显示出分子间接触的晶体包装，在凸起的A1492和A1493与邻近螺旋上的沃森-克里克对的浅槽/小槽之间。在一个晶体中，观察到一个空的A位。在两种晶体中，两种氨基糖苷以不同的方式结合在同一个A位点上，其中一种是特异性结合，另一种是在A位点边缘的深槽中以不同的方式结合。
The crystal structures of six complexes between aminoglycoside antibiotics (neamine, gentamicin C1A, kanamycin A, ribostamycin, lividomycin A and neomycin B) and oligonucleotides containing the decoding A site of bacterial ribosomes are reported at resolutions between 2.2 and 3.0 Å. Although the number of contacts between the RNA and the aminoglycosides varies between 20 and 31, up to eight direct hydrogen bonds between rings I and II of the neamine moiety are conserved in the observed complexes. The puckered sugar ring I is inserted into the A site helix by stacking against G1491 and forms a pseudo base pair with two H-bonds to the Watson–Crick sites of the universally conserved A1408. This central interaction helps to maintain A1492 and A1493 in a bulged-out conformation. All these structures of the minimal A site RNA complexed to various aminoglycosides display crystal packings with intermolecular contacts between the bulging A1492 and A1493 and the shallow/minor groove of Watson–Crick pairs in a neighbouring helix. In one crystal, one empty A site is observed. In two crystals, two aminoglycosides are bound to the same A site with one bound specifically and the other bound in various ways in the deep/major groove at the edge of the A sites.
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影响因子：
3.2
作者：
Pfister, P;Hobbie, S;Westhof, E
通讯作者：
Westhof, E
影响因子：
56.9
作者：
Ogle, JM;Brodersen, DE;Ramakrishnan, V
通讯作者：
Ramakrishnan, V
DOI：
10.1073/pnas.081082398
发表时间：
2001-04-24
影响因子：
11.1
作者：
Nissen, P;Ippolito, JA;Steitz, TA
通讯作者：
Steitz, TA
DOI：
10.1107/s0907444998003254
发表时间：
1998-09-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Brunger, AT;Adams, PD;Warren, GL
通讯作者：
Warren, GL
影响因子：
64.5
作者：
Ogle, JM;Murphy, FV;Ramakrishnan, V
通讯作者：
Ramakrishnan, V