Hooking horseradish peroxidase by using the affinity Langmuir-Blodgett technique for an oriented immobilization
Hooking horseradish peroxidase by using the affinity Langmuir-Blodgett technique for an oriented immobilization
复制标题
使用 Langmuir-Blodgett 亲和技术钩住辣根过氧化物酶进行定向固定
DOI：
10.1016/j.apsusc.2016.11.237
复制
发表时间：
2017-05
影响因子：
6.7
通讯作者：
Huang Xiao Jun
中科院分区：
文献类型：
--
作者：
Ye Peng;Hu Ling Ling;Du Yu Zhi;Xu Yong Juan;Ni Hua Gang;Chen Cong;Lu Xiao Lin;Huang Xiao Jun
A novel method of oriented immobilization was presented: affinity Langmuir-Blodgett (LB) technique. Firstly, a long carbon chain was bond to a ligand of Horseradish Peroxidase (HRP). The ligand derivative appears surface activity with the hydrophobic carbon chain oriented to air and the hydrophilic ligand faced to water. Then, this derivative was put onto the water/air surface to assemble a LB film and formed the affinity interaction with the active site of HRP. After that, the affinity LB film with the enzyme was transferred onto the support to obtain the oriented immobilized HRP. The specific activity of HRP immobilized by affinity LB (182.1 ± 14 U/mg) was higher than that by adsorption (40.5 ± 5 U/mg). HRP immobilized by affinity LB could maintain a more native conformation, compared to that by adsorption. This method could be effectively used to immobilize protein with orientation and show widely promising applications in many fields including biosensor and bioreactor.
登录
查看更多内容
影响因子：
3.3
作者：
Wang, J;Chen, XY;Chen, Z
通讯作者：
Chen, Z
影响因子：
6.7
作者：
Shicheng Xu;A. Dadlani;S. Acharya;P. Schindler;F. Prinz
通讯作者：
Shicheng Xu;A. Dadlani;S. Acharya;P. Schindler;F. Prinz
影响因子：
19
作者：
Wen He;D. Min;Xudong Zhang;Yang Zhang;Zhiying Bi;Y. Yue
通讯作者：
Wen He;D. Min;Xudong Zhang;Yang Zhang;Zhiying Bi;Y. Yue
影响因子：
15
作者：
Jie Wang;M. Even;Xiaoyun Chen;A. Schmaier;J. Waite;Zhan Chen
通讯作者：
Jie Wang;M. Even;Xiaoyun Chen;A. Schmaier;J. Waite;Zhan Chen
影响因子：
3.9
作者：
Baoukina, Svetlana;Monticelli, Luca;Tieleman, D. Peter
通讯作者：
Tieleman, D. Peter