Generation of a Single-Cell RNAseq Atlas of Murine Salivary Gland Development.
Generation of a Single-Cell RNAseq Atlas of Murine Salivary Gland Development.
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DOI：
10.1016/j.isci.2020.101838
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发表时间：
2020-12-18
期刊：
影响因子：
5.8
通讯作者：
Chibly AM
中科院分区：
文献类型：
--
作者：
Hauser BR;Aure MH;Kelly MC;Genomics and Computational Biology Core;Hoffman MP;Chibly AM
了解器官发育过程中的动态转录情况将为再生治疗提供模板。在这里，我们制作了一张小鼠颌下腺的单细胞RNA测序图谱，鉴定了转录图谱，揭示了具有里程碑意义的发育事件：末端芽形成、分支形态发生、细胞分化、成熟和内稳态期间的细胞异质性。轨迹推断分析表明腺泡和导管种群之间是可塑性的。我们鉴定了与腺泡分化相关的转录因子，包括SPDEF、ETV1和XBP1，以及YBX1、Eno1、Sox11和ATF4的缺失。此外，我们描述了由GFRA3和Kit或GSTT1定义的两个插管种群。这一图谱可用于研究特定的细胞功能和预测分支器官发育的共同机制的比较研究。生成的E12、E14、E16、P1、P30和成人SMG Smgc和Bpifa2的scRNAseq图谱定义了与腺泡表型相关的YBX1、eno1、sox11和ATF4的发展中SMG丢失的两个腺泡种群GSTT1定义Kit阴性和性别二态的夹层导管细胞生物科学；发育生物学；系统生物学；转录组学
Understanding the dynamic transcriptional landscape throughout organ development will provide a template for regenerative therapies. Here, we generated a single-cell RNA sequencing atlas of murine submandibular glands identifying transcriptional profiles that revealed cellular heterogeneity during landmark developmental events: end bud formation, branching morphogenesis, cytodifferentiation, maturation, and homeostasis. Trajectory inference analysis suggests plasticity among acinar and duct populations. We identify transcription factors correlated with acinar differentiation including Spdef, Etv1, and Xbp1, and loss of Ybx1, Eno1, Sox11, and Atf4. Furthermore, we characterize two intercalated duct populations defined by either Gfra3 and Kit, or Gstt1. This atlas can be used to investigate specific cell functions and comparative studies predicting common mechanisms involved in development of branching organs. Generated scRNAseq atlas of E12, E14, E16, P1, P30, and adult SMG Smgc and Bpifa2 define two proacinar populations in the developing SMG Loss of Ybx1, Eno1, Sox11, and Atf4 associated with acinar phenotype Gstt1 defines Kit-negative and sexually dimorphic intercalated duct cells Biological Sciences; Developmental Biology; Systems Biology; Transcriptomics
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