Mesenchymal progenitor cells derived from traumatized human muscle.
Mesenchymal progenitor cells derived from traumatized human muscle.
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DOI：
10.1002/term.149
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发表时间：
2009-02
影响因子：
3.3
通讯作者：
Tuan, R. S.
中科院分区：
文献类型：
--
作者：
Jackson, W. M.;Aragon, A. B.;Djouad, F.;Song, Y.;Koehler, S. M.;Nesti, L. J.;Tuan, R. S.
关键词：
来源于成人组织的间充质干细胞（MSCs）是基于细胞的组织工程和再生医学的重要候选细胞类型。目前，骨髓间充质干细胞的临床应用需要额外的外科手术来获取自体骨髓间充质干细胞（即来自骨髓）或商业同种异体替代品。我们最近在外科手术清除的创伤性矫形肢体伤口的创伤性肌肉组织中发现了间充质祖细胞（MPCs）群体。本研究的目的是通过比较创伤性肌肉来源的骨髓间充质干细胞的形态、增殖能力、细胞表面表位谱和分化能力，来评估它们是否可以作为骨髓间充质干细胞的临床替代品。用胶原酶消化肌肉组织后，用直接镀技术富集MPCs细胞。肌源性骨髓间充质干细胞的形态和增殖速率与骨髓源性骨髓间充质干细胞相似。这两个群体都表达了MSCs特有的细胞表面标记（cd73、cd90和CD105），而不表达MSCs通常不存在的标记（CD14、CD34和CD45）。在特定分化培养基中培养21天后，MPCs和MSCs的组织学染色和基因表达表现出向成骨细胞、软骨细胞和脂肪细胞分化的特征，但未向成肌细胞分化。我们的研究结果表明，创伤后的肌肉来源的骨髓间充质干细胞表现出相似的表型，与骨髓来源的骨髓间充质干细胞相似。创伤性肌肉组织中提取的MPCs可用于骨科创伤后需要周清创的组织工程和再生医学。
Mesenchymal stem cells (MSCs) derived from adult tissues are an important candidate cell type for cell-based tissue engineering and regenerative medicine. Currently, clinical applications for MSCs require additional surgical procedures to harvest the autologous MSCs (i.e., from bone marrow) or commercial allogeneic alternatives. We have recently identified a population of mesenchymal progenitor cells (MPCs) in traumatized muscle tissue that has been surgically debrided from traumatic orthopaedic extremity wounds. The purpose of this study was to evaluate whether MPCs derived from traumatized muscle may provide a clinical alternative to bone-marrow MSCs by comparing their morphology, proliferation capacity, cell surface epitope profile and differentiation capacity. After digesting the muscle tissue with collagenase, the MPCs cells were enriched by a direct plating technique. The morphology and proliferation rate of the muscle-derived MPCs was similar to bone-marrow derived MSCs. Both populations expressed cell surface markers characteristic for MSCs (CD 73, CD 90 and CD105), and did not express markers typically absent on MSCs (CD14, CD34 and CD45). After 21 days in specific differentiation media, the histological staining and gene expression of the MPCs and MSCs was characteristic for differentiation into osteoblasts, chondrocytes and adipocytes but not into myoblasts. Our findings demonstrate that traumatized muscle-derived MPCs exhibit similar phenotype and resemble MSCs derived from the bone marrow. MPCs harvested from traumatized muscle tissue may be considered for applications in tissue engineering and regenerative medicine following orthopaedic trauma requiring circumferential debridement.
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影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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DOI：
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