Egg components reverse the atrophy induced by injury in skeletal muscles
Egg components reverse the atrophy induced by injury in skeletal muscles
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鸡蛋成分可逆转骨骼肌损伤引起的萎缩
DOI：
10.1111/gtc.12915
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发表时间：
2022
期刊：
影响因子：
2.1
通讯作者：
Mizunoya Wataru
中科院分区：
文献类型：
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作者：
Sawano Shoko;Kobayashi Yuya;Maesawa Suzuka;Mizunoya Wataru
骨骼肌萎缩是由诸如不活动、营养不良、衰老和损伤等因素引起的肌肉组织损失。在这项研究中，我们的目的是调查鸡蛋成分是否发挥抑制肌肉萎缩的作用。将鸡蛋混合物溶液连续10天口服给药于在胫骨前肌（TA）肌肉中注射心脏毒素的雄性C57 BL/6 J小鼠。通过蛋白质印迹法观察到，鸡蛋混合物的给药显著降低了atrogin-1和MuRF-1蛋白水平，这是肌肉萎缩的关键因素。此外，我们研究了蛋成分如抗生物素蛋白、卵磷脂、生物素、3-sn-磷脂酰胆碱和L-α-磷脂酰胆碱对地塞米松（DEX）处理的C2 C12肌管的影响。蛋黄中的卵磷脂、生物素、3-sn-磷脂酰胆碱和L-α-磷脂酰胆碱显著恢复了DEX应用后减少的C2 C12肌管直径。抗生物素蛋白没有显示这种逆转。生物素、3-sn-磷脂酰胆碱和L-α-磷脂酰胆碱也减弱了DEX增强的atrogin-1蛋白表达。我们的研究结果表明，蛋黄成分可能有助于逆转骨骼肌损伤引起的萎缩。
Skeletal muscle atrophy is the loss of muscle tissue caused by factors such as inactivity, malnutrition, aging, and injury. In this study, we aimed to investigate whether egg components exert inhibitory effects on muscle atrophy. An egg mix solution was orally administered for 10 consecutive days to male C57BL/6J mice injected with cardiotoxin in the tibialis anterior (TA) muscle. The administration of egg mixture significantly decreased the atrogin‐1 and MuRF‐1 protein levels, key factors in muscle atrophy, as observed by western blotting. Furthermore, we investigated the effects of egg components such as avidin, lecithin, biotin, 3‐sn‐phosphatidylcholine, and L‐α‐phosphatidylcholine on dexamethasone (DEX)‐treated C2C12 myotubes. Lecithin, biotin, 3‐sn‐phosphatidylcholine, and L‐α‐phosphatidylcholine in egg yolk significantly recovered the diameters of C2C12 myotubes decreased upon DEX application. Avidin did not show such reversal. Biotin, 3‐sn‐phosphatidylcholine, and L‐α‐phosphatidylcholine also attenuated atrogin‐1 protein expression enhanced by DEX. Our findings reveal that egg yolk components could contribute to the reversal of skeletal muscle atrophy induced by muscle injury.
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影响因子：
6.1
作者：
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通讯作者：
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影响因子：
3.3
作者：
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29
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通讯作者：
Donovan A
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影响因子：
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作者：
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通讯作者：
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