The impact of genetic modified Ma bamboo on soil microbiome.
The impact of genetic modified Ma bamboo on soil microbiome.
复制标题
转基因麻竹对土壤微生物组的影响
DOI：
10.3389/fmicb.2022.1025786
复制
发表时间：
2022
影响因子：
5.2
通讯作者：
Zhu, Qiang
中科院分区：
文献类型：
--
作者：
Wang, Kai;Liu, Mengxia;Cai, Changyang;Cai, Shifeng;Ma, Xiangqing;Lin, Chentao;Zhu, Qiang
评价转基因植物微生物群落的潜在变化是保证转基因植物生物安全性的重要环节。最近，我们已经产生了转基因的麻竹与增加耐寒性和耐旱性的花青素积累。本研究旨在研究转基因竹栽培对根际土壤微生物群落的潜在影响。在移植后3个月收集Rbesphere和周围土壤。对扩增子（16 S rDNA和ITS 1）进行测序以分析细菌和真菌群落。应用多种软件和数据库（Picrust 2、FAPROTAX和FUNGulid）预测和比较微生物功能，包括基本代谢、氮利用和植物病原体的存在。转基因竹与野生竹根际土壤微生物群落结构和丰度没有明显变化。对于周围土壤，细菌生物区系α-多样性增加（chao 1：1，001 ± 80- 1，276 ± 84，观察种：787 ± 52- 1，194 ± 137，PD全树：75 ± 4-117 ± 18），真菌生物群α多样性下降（chao1：187 ± 18-145 ± 10）。转基因竹与野生竹根际和周围土壤中的微生物群预测功能没有改变或没有负向改变。结果表明，转基因竹生长对根际土壤微生物群落没有实质性影响，对竹周土壤微生物群落影响较小，未造成有害影响。此外，转基因竹子对土壤微生物区系的预测功能没有负面影响。
Evaluating the potential alteration of microbial communities is a vital step for biosafety of genetic modified plants. Recently, we have produced genetic modified Ma bamboo with increased cold and drought tolerance by anthocyanin accumulation. In this work, we aim to study the potential effects on microbial communities in rhizosphere soils during the cultivation of genetic modified bamboo. Rhizosphere and surrounding soil were collected at 3-month post-transplant. The amplicon (16S rDNA and ITS1) were sequenced for analysis of bacterial and fungal communities. Multiple software and database (Picrust2, FAPROTAX and FUNGulid) were applied to predict and compare the microbial functions involving basic metabolisms, nitrogen usage and presence of plant pathogens. There were no substantial change of the structure and abundance of rhizosphere soil microbial communities between genetic modified and wild type bamboo. For the surrounding soil, the bacterial biota α-diversity increased (chao1: 1,001 ± 80–1,276 ± 84, observed species: 787 ± 52–1,194 ± 137, PD whole tree: 75 ± 4–117 ± 18) and fungal biota α-diversity decreased (chao1: 187 ± 18–145 ± 10) in samples of genetic modified bamboo compared to those of wild type bamboo. The microbiota predicted functions did not change or had no negative alteration between genetic modified and wild type bamboo, in both rhizosphere and surrounding soils. As a conclusion, the growth of genetic modified bamboo had no substantial change on rhizosphere soil microbial communities, while minor alteration on bamboo surrounding soil microbial communities with no harmful effects. Moreover, the genetic modified bamboo had no negative effect on the predicted functions of microbiota in soil.
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