Insertional inactivation of a fowlpox virus homologue of the vaccinia virus F12L gene inhibits the release of enveloped virions.
Insertional inactivation of a fowlpox virus homologue of the vaccinia virus F12L gene inhibits the release of enveloped virions.
复制标题
痘苗病毒 F12L 基因的鸡痘病毒同源物的插入失活可抑制有包膜病毒颗粒的释放。
DOI：
10.1099/0022-1317-74-1-55
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Keyvan Nazerian
中科院分区：
文献类型：
--
作者：
R. Ogawa;Jay G. Calvert;N. Yanagida;Keyvan Nazerian
将大肠杆菌 lacZ 基因插入鸡痘病毒 (FPV) 基因组 5.7 kb HindIII 片段的 ClaI 限制性酶切位点，从而产生稳定的重组体。这些重组体产生的噬斑明显小于亲本 FPV 或在其他非必需位点含有 lacZ 基因的 FPV 重组体产生的噬斑。将外源 DNA 插入 ClaI 位点会破坏先前未识别的开放阅读框 (ORF)，该框可能编码 74K 多肽。该FPV ORF的预测氨基酸序列与痘苗病毒的F12L ORF有24%的同一性，但其功能目前尚不清楚。感染重组病毒或亲本病毒的细胞中胞内 FPV 的产生相似，但重组体释放到培养基中的感染性胞外病毒粒子数量约为亲本病毒释放的感染性胞外病毒粒子数量的 20%。同样，体内用[3H]胸苷标记的FPV颗粒的释放在重组FPV感染的细胞中显着降低。这些结果表明痘苗病毒 F12L ORF 的 FPV 同源物参与感染性细胞外病毒粒子的包封或释放。
Insertion of the Escherichia coli lacZ gene into a ClaI restriction enzyme site of a 5.7 kb HindIII fragment of the fowlpox virus (FPV) genome resulted in the generation of stable recombinants. These recombinants produced plaques that were significantly smaller than those produced by parental FPV or by FPV recombinants containing the lacZ gene at other non-essential sites. Insertion of foreign DNA into the ClaI site disrupts a previously unidentified open reading frame (ORF) which potentially encodes a 74K polypeptide. The predicted amino acid sequence of this FPV ORF has 24% identity with the F12L ORF of vaccinia virus, the function of which is not currently known. Production of intracellular FPV was similar in cells infected with recombinant or parental viruses, but the number of infectious extracellular virions released into the medium by the recombinant was about 20% of that released by the parental virus. Likewise, the release of FPV particles, which were labelled in vivo with [3H]thymidine, was significantly lower in recombinant FPV-infected cells. These results suggest that the FPV homologue of the vaccinia virus F12L ORF is involved in the envelopment or release of infectious extracellular virions.
影响因子：
5.5
作者：
Jill Taylor;R. Weinberg;Y. Kawaoka;R. G. Webster;Enzo Paoletti;Enzo Paoletti
通讯作者：
Jill Taylor;R. Weinberg;Y. Kawaoka;R. G. Webster;Enzo Paoletti;Enzo Paoletti