Human isolated ileum: motor responses of the circular muscle to electrical field stimulation and exogenous neuropeptides
Human isolated ileum: motor responses of the circular muscle to electrical field stimulation and exogenous neuropeptides
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人离体回肠：环形肌对电场刺激和外源神经肽的运动反应
DOI：
10.1007/bf00169740
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
A. Meli
中科院分区：
文献类型：
--
作者：
C. Maggi;R. Patacchini;P. Santicioli;S. Giuliani;D. Turini;G. Barbanti;A. Giachetti;A. Meli
总结（1）来自人回肠的环形定向肌条对电场刺激（1-50 Hz）作出反应，在低频下具有频率相关的初级松弛，在高频刺激下具有初级收缩。河豚毒素（1 μM）可消除或显著降低这两种反应。(2)阿托品（3 μ M）或ω-芋螺毒素（0.1 μM）可减弱但不消除电场刺激引起的收缩，并增强其舒张作用。ω芋螺毒素（0.1 μM）不影响卡巴胆碱诱导的收缩或异丙肾上腺素诱导的舒张。(3)神经激肽A和P物质（1 nM-1 μM）产生浓度依赖性收缩。NK-1受体选择性激动剂[Pro 9]SP sulfone和NK-2受体选择性激动剂[β Ala 8]NKA（4-10）分别产生与P物质和神经激肽A重叠的收缩。另一方面，[MePhe 7]-神经激肽B，一种NK-3受体选择性激动剂，在高达1 [M]时无效。阿托品（3 μM）不影响对P物质或神经激肽A的反应。(4)甘丙肽，高达0.1 μM产生弱的和不一致的收缩。(5)血管活性肠肽（10 nM - 1 μM）产生浓度依赖性舒张，而人α降钙素基因相关肽产生弱且不一致的舒张作用。(6)这些结果表明，胆碱能兴奋性和非胆碱能抑制性神经都影响人小肠环行肌的运动。兴奋性神经释放的递质似乎主要是通过激活ω芋螺毒素敏感性（N型）钙通道介导的。速激肽通过NK-1和NK-2受体独立于胆碱能神经发挥有效的收缩作用。
Summary(1) Circularly-oriented muscle strips from the human ileum responded to electrical field stimulation (1–50 Hz) with frequency-related primary relaxation at low frequency and primary contractions at high frequencies of stimulation. Both responses were abolished or markedly reduced by tetrodotoxin (1 μM). (2) Atropine (3 μ M) or omega conotoxin (0.1 μM) reduced but dit not abolish contraction to electrical field stimulation and enhanced the relaxation. Omega conotoxin (0.1 μM) did not affect carbachol-induced contraction nor isoprenaline-induced relaxation. (3) Neurokinin A and substance P (1 nM-1 μM) produced a concentration-dependent contraction. The NK-1 receptor selective agonist, [Pro9]SP sulfone and the NK-2 receptor selective agonist [βAla8]NKA(4-10) prodneed a contraction superimposable to that of substance P and neurokinin A, respectively. On the other hand, [MePhe7]-neurokinin B, an NK-3 receptor selective agonist was ineffective up to 1 [M. The response to substance P or neurokinin A was unaffected by atropine (3 μM). (4) Galanin, up to 0.1 μM produced a weak and inconsistent contraction. (5) Vasoactive intestinal polypeptide (10 nM - 1 μM) produced a concentration-dependent relaxation while human alpha calcitonin gene-related peptide exerted a weak and inconsistent relaxant effect. (6) These findings indicate that both cholinergic excitatory and non-cholinergic inhibitory nerves affect the motility of the circular muscle of the human small intestine. Transmitter release from excitatory nerves seems largely mediated by activation of omega conotoxin-sensitive (N-type) calcium channels. Tachykinins exert a potent contractile effect independently of cholinergic nerves via NK-1 and NK-2 receptors.
血管活性肠多肽：犬胃粘膜肌层中的假定递质。
DOI：
10.1113/jphysiol.1983.sp014830
发表时间：
1983
期刊：
The Journal of physiology
影响因子：
--
作者：
Angel,F;Go,VL;Schmalz,PF;Szurszewski,JH
通讯作者：
Szurszewski,JH
影响因子：
5.5
作者：
FOX, AP;NOWYCKY, MC;TSIEN, RW
通讯作者：
TSIEN, RW