Potential of Single Cell Gel Electrophoresis Assay (Comet Assay) in Heavy Ion Radiation Biology
Potential of Single Cell Gel Electrophoresis Assay (Comet Assay) in Heavy Ion Radiation Biology
复制标题
单细胞凝胶电泳测定（彗星测定）在重离子辐射生物学中的潜力
DOI：
--
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
R. Saran
中科院分区：
文献类型：
--
作者：
Y. R. Ahuja;R. Saran
Abstract DNA damage and repair following heavy ion radiation have been studied using various techniques like pulse field gel electrophoresis, sedimentation, and neutral/ alkali elution. These techniques are cumbersome, time consuming and need a substantial cell-sample size. In addition, these techniques are unable to identify differentially, highly damaged or apoptotic subpopulation of cells, a characteristic result of exposure of heavy ion radiation, since the results are based on a pooled population of cells. Most of these difficulties can be overcome by using single cell gel electrophoresis assay, commonly known as comet assay. For this assay, a small cell-sample size (a few thousand cells) is required, and the results are available in a short time (5-6 hours). Since, the information on DNA damage and repair is available on individual cells, any subpopulation of cells with a different response like high/low DNA damage or apoptosis can be identified. Moreover, this assay is adept in evaluating SSBs., DSBs, alkali labile sites, adduct formation, cross-linking (DNA-DNA or DNA-protein) and base damage. Not only that, damage to specific region of chromosome may be detected using FISH in combination with comet assay. Hence, the use of comet assay may have potential in the estimation of DNA damage and repair in heavy ion radiation biology.
登录
查看更多内容
单细胞凝胶电泳/彗星测定：检测人类辐射引起的 DNA 损伤的潜在工具。
DOI：
--
发表时间：
1995
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Tice,RR;Strauss,GH
通讯作者：
Strauss,GH
褪黑激素：其“辐射防护”作用的可能机制。
DOI：
10.1016/s0027-5107(98)00112-2
发表时间：
1998
期刊：
Mutation research
影响因子：
--
作者：
Vijayalaxmi;Reiter,RJ;Meltz,ML;Herman,TS
通讯作者：
Herman,TS
60 Hz 磁场暴露会诱导大鼠脑细胞中的 DNA 交联。
DOI：
10.1016/s0027-5107(98)00017-7
发表时间：
1998
期刊：
Mutation research
影响因子：
--
作者：
Singh,N;Lai,H
通讯作者：
Lai,H
影响因子：
11.2
作者：
Peggy L. Olive;Ralph E. Durand;J. L. Riche;Ivo A. Olivotto;Stewart M. Jackson
通讯作者：
Peggy L. Olive;Ralph E. Durand;J. L. Riche;Ivo A. Olivotto;Stewart M. Jackson