Monitoring of circulating tumour-associated DNA as a prognostic tool for oral squamous cell carcinoma.
Monitoring of circulating tumour-associated DNA as a prognostic tool for oral squamous cell carcinoma.
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DOI：
10.1038/sj.bjc.6602635
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发表时间：
2005-06-20
影响因子：
8.8
通讯作者：
Tanzawa, H
中科院分区：
文献类型：
--
作者：
Hamana, K;Uzawa, K;Ogawara, K;Shiiba, M;Bukawa, H;Yokoe, H;Tanzawa, H
常见的等位基因失衡（AIs），包括杂合性缺失和特定染色体区域的微卫星不稳定性，已经在各种人类恶性肿瘤中被发现。本研究的目的是通过微卫星血液检测评估口腔鳞状细胞癌（SCC）预后和监测的可能性。在9个微卫星位点检测64例口腔SCC患者的正常组织和肿瘤组织的DNA以及在三个时间点（术前、术后和术后4周）获得的血清DNA。总的来说，38份（59%）来自肿瘤组织的DNA样本和52%来自血清的DNA样本至少在一个位点显示AIs。血清DNA中AIs的模式与肿瘤DNA中检测到的模式相匹配。其中，术前（44%，64例中28例）和术后（20%，64例中13例）经常检测到ai。12例术后发生AI的患者中，6例术后4周无AI迹象，无复发，无疾病。相比之下，术后4周ai阳性DNA的6例患者在44周内死于远处转移。因此，我们的研究结果表明，评估血清DNA中的微卫星状态可能是监测疾病预后的有用预测工具。
Frequent allelic imbalances (AIs) including loss of heterozygosity and microsatellite instability on a specific chromosomal region have been identified in a variety of human malignancies. The objective of our study was to assess the possibility of prognostication and monitoring of oral squamous cell carcinoma (SCC) by microsatellite blood assay. DNA from normal and tumorous tissues and serum DNA obtained at three time points (preoperatively, postoperatively, and 4 weeks postoperatively) from 64 patients with oral SCC was examined at nine microsatellite loci. In all, 38 (59%) DNA samples from tumorous tissues and 52% from serum showed AIs in at least one locus. Patterns of AIs in the serum DNA were matched to those detected in tumour DNA. Of them, AIs were frequently detected preoperatively (44%, 28 of 64), and postoperatively (20%, 13 of 64). Moreover, among 12 cases with AIs during the postoperative period, six had no evidence of an AI 4 weeks postoperatively, and they had no recurrence and were disease free. In contrast, six patients with AI-positive DNA 4 weeks postoperatively have died with distant metastasis within 44 weeks. Thus, our results suggest that the assessment of microsatellite status in the serum DNA could be a useful predictive tool to monitor disease prognosis.
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