Transcriptome Sequencing reveals the expressed profiles of mRNA and ncRNAs and regulate network via ceRNA mediated molecular mechanism of lung adenocarcinoma bone metastasis in Xuanwei.
Transcriptome Sequencing reveals the expressed profiles of mRNA and ncRNAs and regulate network via ceRNA mediated molecular mechanism of lung adenocarcinoma bone metastasis in Xuanwei.
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转录组测序揭示了mRNA和NCRNA的表达曲线，并通过CERNA介导的肺腺癌骨转移的分子机制调节网络。
DOI：
10.21037/tcr-20-2376
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发表时间：
2021-01
影响因子：
0.9
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Han L;Yao Z;Xie L;Li D;Wang C;Yang Y;Yang J;Huang Z;Li K;Zhang Y;Ye L;Tan Z;Liu Y;Chen Q;Wang T;Yang Z
肺癌最常见的亚型是肺腺癌，其特点是转移能力强。宣威地区LuAC发生率高，预后差，死亡率高。本研究采用转录组测序的方法，系统地探讨了宣威LuAC骨转移的分子机制。采用RNA测序的方法研究非编码RNA(NcRNAs)在LuAC和LuAC骨转移瘤中的表达谱。我们鉴定了差异表达的mRNAs(DEmRNAs)、miRNAs(DEmiRNAs)、lncRNAs(DElncRNAs)和CircRNAs(DECircRNAs)。生物信息学分析了LuAC骨转移相关竞争内源RNA(CERNA)可能的关系和功能。采用定量逆转录聚合酶链式反应(qRT-PCR)检测这些差异表达基因在血清中的表达。在宣威原发LuAC和LuAC骨转移患者中分别检测到2141个DEmRNAs、43个DEmiRNAs、136个DElncRNAs和706个DECircRNAs。本研究构建了宣威转移瘤LuAC的CircRNA/miRNA/mRNAs和lncRNA/miRNA/mRNAs网络，通过CERNA调控网络揭示了ncRNAs调控基因表达的机制。我们观察到lncRNA(ADAMTS9-AS2、TEX41、DLEU2、LINC00152)-miR-223-3p-SCARB1和hsa_circ_0000053-miR-196a-5p/miR-196b-5p-HOXA5 CENA网络在宣威陆癌骨转移中可能起重要作用。我们全面鉴定了宣威LuAC在骨转移中的CENA调控网络，并揭示了不同ncRNAs表达谱的贡献。我们的研究结果表明，在宣威LuAC的骨转移机制中，mRNAs和ncRNAs之间存在关联。
The most ordinary subtype of lung cancer is lung adenocarcinoma (LuAC), which is characterized by strong metastatic ability. And LuAC rates in Xuanwei leads to the poor prognosis and high death rate. In this study, we systematically explored the molecular mechanism of LuAC bone metastasis in Xuanwei by transcriptome sequencing. RNA Sequencing was conducted to explore the noncoding RNAs (ncRNAs) expression profiles in primary LuAC and LuAC bone metastasis. We identified differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs), lncRNAs (DElncRNAs) and circRNAs (DEcircRNAs). Bioinformatics analyses the possible relationships and functions of the LuAC bone metastasis-related competing endogenous RNA (ceRNA). And qRT-PCR was performed to evaluate the expression of these differently expressed genes in serum. A total of 2,141 DEmRNAs, 43 DEmiRNAs, 136 DElncRNAs and 706 DEcircRNAs were identified in the Xuanwei patients with primary LuAC vs. LuAC bone metastasis, respectively. The circRNA/miRNA/mRNA and lncRNA/miRNA/mRNA networks of LuAC in Xuanwei with bone metastasis were built, and the gene expression mechanisms regulated by ncRNAs were unveiled via the ceRNA regulatory networks. We observe that lncRNA (ADAMTS9-AS2, TEX41, DLEU2, LINC00152)-miR-223-3p-SCARB1 and hsa_circ_0000053-miR-196a-5p/miR-196b-5p-HOXA5 ceRNA networks might play an important role in bone metastasis of Xuanwei LuAC. We comprehensively identified ceRNA regulatory networks of LuAC in Xuanwei with bone metastasis as well as revealed the contribution of different ncRNAs expression profiles. Our data demonstrate the association between mRNAs and ncRNAs in the metastasis mechanism of LuAC in Xuanwei with bone metastasis.
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影响因子：
--
作者：
Guerriero I;D'Angelo D;Pallante P;Santos M;Scrima M;Malanga D;De Marco C;Ravo M;Weisz A;Laudanna C;Ceccarelli M;Falco G;Rizzuto A;Viglietto G
通讯作者：
Viglietto G
DOI：
10.1042/bj20111451
发表时间：
2012-01-15
期刊：
The Biochemical journal
影响因子：
--
作者：
Lee J;Giordano S;Zhang J
通讯作者：
Zhang J
影响因子：
4.5
作者：
Huang, Guoren;Xue, Jinli;Yu, Liangli (Lucy)
通讯作者：
Yu, Liangli (Lucy)
影响因子：
20.4
作者：
Goldstraw, Peter;Chansky, Kari;Bolejack, Vanessa
通讯作者：
Bolejack, Vanessa
竞争性内源性 RNA 网络与胃癌
影响因子：
4.3
作者：
Guo, Lei-Lei;Song, Chun-Hua;Wang, Kai-Juan
通讯作者：
Wang, Kai-Juan