Characterization and primary structure of a human immunodeficiency virus type 1 (HIV-1) neutralization domain as presented by a poliovirus type 1/HIV-1 chimera.
Characterization and primary structure of a human immunodeficiency virus type 1 (HIV-1) neutralization domain as presented by a poliovirus type 1/HIV-1 chimera.
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1 型脊髓灰质炎病毒/HIV-1 嵌合体所呈现的 1 型人类免疫缺陷病毒 (HIV-1) 中和结构域的特征和一级结构。
DOI：
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发表时间：
1993
影响因子：
3.8
通讯作者：
P. D. Minor
中科院分区：
文献类型：
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作者：
Cherelyn Vella;Morag Ferguson;G. Dunn;R. H. Meloen;H. Langedijk;David H. Evans;P. D. Minor
脊髓灰质炎病毒/人类免疫缺陷病毒(HIV)嵌合体S1/env/3呈现序列DRPEGIEEEGGERDRDRS，这是一种已知的HIV IIIB糖蛋白gp41中和结构域(残基735至752)，位于脊髓灰质炎病毒1型萨宾株的抗原位点。在S1/env/3中提出的10个针对该序列的单抗中，有8个在体外被证明中和了HIV IIIB，而所有10个都中和了S1/env/3，这表明该序列的呈现在HIV和脊髓灰质炎病毒/HIV嵌合体之间是相似的。通过从S1/env/3中筛选逃逸突变体和PepScan分析，鉴定了单抗的特性。这两种方法给出了类似的结果，确定了两个表位，涉及对应于gp41的740至743位和745至750位的氨基酸。在具有S1/env/3特异性单抗的嵌合体中选择的突变与HIV-1自然分离株体内发生的变化相同或相似。这一发现表明，该表位可能在体内中和HIV方面具有重要意义。
The poliovirus/human immunodeficiency virus (HIV) chimera S1/env/3 presents the sequence DRPEGIEEEGGERDRDRS, a known glycoprotein gp41 neutralizing domain (residues 735 to 752) of HIV IIIB in an antigenic site of the Sabin type 1 strain of poliovirus. Of 10 monoclonal antibodies raised against the sequence as presented in S1/env/3, eight were shown to neutralize HIV IIIB in vitro whereas all 10 neutralized S1/env/3, suggesting that the presentation of the sequence is comparable between HIV and the poliovirus/HIV chimera. The monoclonal antibodies were characterized by the selection of escape mutants from S1/env/3 and by Pepscan analysis. The two methods gave similar results, identifying two epitopes involving amino acids corresponding to residues 740 to 743, and to residues 745 to 750 of gp41. Mutations selected in the chimera with S1/env/3-specific MAbs are identical or similar to changes occurring in vivo in natural isolates of HIV-1. This finding suggests that the epitope may be significant in the neutralization of HIV in vivo.
脊髓灰质炎病毒/鼻病毒抗原杂交体的构建和表征。
影响因子：
3.7
作者：
Altmeyer,R;Murdin,AD;Harber,JJ;Wimmer,E
通讯作者：
Wimmer,E
影响因子：
3.8
作者：
MINOR, PD;FERGUSON, M;ICENOGLE, JP
通讯作者：
ICENOGLE, JP