Single‐Cell Sorting of Microorganisms by Flow or Slide‐Based (Including Laser Scanning) Cytometry
Single‐Cell Sorting of Microorganisms by Flow or Slide‐Based (Including Laser Scanning) Cytometry
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通过流式或基于载玻片（包括激光扫描）细胞计数法对微生物进行单细胞分选
DOI：
10.1002/1521-3846(200105)21:2
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Y. Tani
中科院分区：
文献类型：
--
作者：
T. Katsuragi;Y. Tani
在应用微生物学中，通过常规选择培养对微生物进行菌株改良并不总是成功的，因此可以使用从大的异质群体中选择具有所需特征的活细胞的单细胞选择。使用显微操作器进行单细胞选择是可能的，但很费力。对于许多应用程序，该过程已经自动化。在这篇综述中，一个自动化的方法，激光扫描细胞术（LSC），概述了与流式细胞术（FCM）。FCM对许多微生物学家来说都很熟悉，但LSC是一种不太为人所知的基于显微镜载玻片的方法。其优点之一是它可能用于检查小细胞群。此外，单个细胞可以重复检查，自动测量，随后由操作员进行显微镜观察，最后储存（如果需要）在放置它们的显微镜载玻片上。荧光和其他探针可用于FCM，几乎所有都可用于LSC。这些方法的一些应用被引用的广泛的文献（主要是关于FCM），但在这篇评论中可能的应用程序的列表是远远不够详尽。这篇评论的目的是为应用微生物学家介绍LSC的多种用途。
In applied microbiology, strain improvement of microorganisms by conventional selection culture is not always successful, so single-cell selection of viable cells with the desired characteristics from a large heterogeneous population may be used instead. Single-cell selection with use of a micromanipulator is possible, but laborious. For many applications, the process has been automated. In this review, an automated method, laser scanning cytometry (LSC), is outlined together with flow cytometry (FCM). FCM is familiar to many microbiologists, but LSC is a microscopic-slide-based method that is less well known. One of its advantages is its possible use in the examination of small cell populations. In addition, individual cells can be examined repeatedly, measured automatically and later observed microscopically by the operator, and finally stored (if desired) on the microscopic slide on which they are placed. Fluorescent and other probes are available in abundance for FCM, and almost all might be used in LSC. A number of applications of these methods are cited from the extensive literature (mostly about FCM), but the list of possible applications in this review is far from being exhaustive. This review is intended as an introduction for the applied microbiologist to the manifold uses of LSC.
与稳定在凝胶微滴中的染色体进行原位杂交。
DOI：
10.1002/cyto.990210202
发表时间：
1995
期刊：
Cytometry.
影响因子：
--
作者：
Nguyen,BT;Lazzari,K;Abebe,J;Mac,I;Lin,JB;Chang,A;Wydner,KL;Lawrence,JB;Cram,LS;Weier,HU
通讯作者：
Weier,HU