Red and problematic green phylogenetic signals among thousands of nuclear genes from the photosynthetic and apicomplexa-related Chromera velia.
Red and problematic green phylogenetic signals among thousands of nuclear genes from the photosynthetic and apicomplexa-related Chromera velia.
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DOI：
10.1093/gbe/evr100
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发表时间：
2011
影响因子：
3.3
通讯作者：
Gould SB
中科院分区：
文献类型：
--
作者：
Woehle C;Dagan T;Martin WF;Gould SB
光合作用和基础apicomplexan Chromera velia最近被描述，扩大了成员，否则非光合组的寄生原生生物。顶复门是一种具有次生质体的泡状藻类，但其核基因的进化历史仍在积极讨论中。使用表达基因的深度测序，我们通过生成具有真核同源物的簇并构建系统发育树和网络来研究3，151个表达序列标签重叠群的严格过滤集的系统发育亲和力。确定了这一蜂窝状突起的系统发育定位，并提取了门特异性蛋白质。系统发育树提供了相互矛盾的信号，有444棵树归为C类。而C. velia与蜂窝牡蛎病原体Perkinsus marinus，后者的信号被加强从共享基因和整体序列相似性的分析。在513 C.在反映光合作用祖先并且其核同源物可从红色和绿色谱系获得的膜细胞核基因中，263个指示红色光合作用祖先，而250个指示绿色光合作用祖先。相同的1：1的信号比之间发现推定的255核编码的质体蛋白质鉴定。蜂窝状体的红色和绿色信号的发现反映了在异鞭毛体谱系中观察到的结果，并支持异鞭毛体和蜂窝状体中质体的共同但不一定是单一起源。在这些谱系中，绿色内共生先于红色质体获得的推断导致了令人担忧的复杂进化情景，促使人们寻找绿色系统发育信号和所涉及宿主数量的其他解释。
The photosynthetic and basal apicomplexan Chromera velia was recently described, expanding the membership of this otherwise nonphotosynthetic group of parasite protists. Apicomplexans are alveolates with secondary plastids of red algal origin, but the evolutionary history of their nuclear genes is still actively discussed. Using deep sequencing of expressed genes, we investigated the phylogenetic affinities of a stringent filtered set of 3,151 expressed sequence tag-contigs by generating clusters with eukaryotic homologs and constructing phylogenetic trees and networks. The phylogenetic positioning of this alveolate alga was determined and sets of phyla-specific proteins extracted. Phylogenetic trees provided conflicting signals, with 444 trees grouping C. velia with the apicomplexans but 354 trees grouping C. velia with the alveolate oyster pathogen Perkinsus marinus, the latter signal being reinforced from the analysis of shared genes and overall sequence similarity. Among the 513 C. velia nuclear genes that reflect a photosynthetic ancestry and for which nuclear homologs were available both from red and green lineages, 263 indicated a red photosynthetic ancestry, whereas 250 indicated a green photosynthetic ancestry. The same 1:1 signal ratio was found among the putative 255 nuclear-encoded plastid proteins identified. This finding of red and green signals for the alveolate mirrors the result observed in the heterokont lineage and supports a common but not necessarily single origin for the plastid in heterokonts and alveolates. The inference of green endosymbiosis preceding red plastid acquisition in these lineages leads to worryingly complicated evolutionary scenarios, prompting the search for other explanations for the green phylogenetic signal and the amount of hosts involved.
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具有复杂红色质体的生物体中的ERAD成分表明，募集了Periplastid膜的蛋白质转运途径。
影响因子：
3.3
作者：
Felsner G;Sommer MS;Gruenheit N;Hempel F;Moog D;Zauner S;Martin W;Maier UG
通讯作者：
Maier UG
影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
影响因子：
14.9
作者：
Aurrecoechea, Cristina;Heiges, Mark;Kissinger, Jessica C.
通讯作者：
Kissinger, Jessica C.
影响因子：
6.8
作者：
AKAIKE, H
通讯作者：
AKAIKE, H
影响因子：
2.2
作者：
Cavalier-Smith, T
通讯作者：
Cavalier-Smith, T