Distinct sets of Rab6 effectors contribute to ZW10--and COG-dependent Golgi homeostasis.
Distinct sets of Rab6 effectors contribute to ZW10--and COG-dependent Golgi homeostasis.
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DOI：
10.1111/tra.12167
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发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Storrie B
中科院分区：
文献类型：
--
作者：
Majeed W;Liu S;Storrie B
高尔基体的组织部分是由Rab蛋白及其多种效应物的相互作用决定的。在这里，我们使用多种方法来识别和表征一小部分效应器，这些效应器模仿Rab6对高尔基带组织的影响。在基于视觉的候选蛋白筛选中，我们发现三个Rab6效应物，肌球蛋白IIA (MyoIIA)， Kif20A和Bicaudal D （BicD）中的任何一个的个体消耗都足以抑制高尔基带的断裂/分散，这种方式与Rab6平行，与逆行系绳蛋白耦合。MyoIIA和Kif20A缺失具有通路选择性，仅抑制ZW10依赖性高尔基带的断裂/扩散，而BicD缺失与Rab6一样，抑制ZW10依赖性和cog依赖性高尔基带的断裂。可逆肌球蛋白抑制剂blebbistatin可在短期试验中产生MyoIIA效应。在电子显微镜水平上，bcd缺失的影响与rab6缺失的影响相似：高尔基池更长、更连续，包被囊泡明显积聚。在功能上，bbicd缺失的细胞被抑制了新合成的VSV-G蛋白向细胞表面的运输。总之，我们的研究结果表明，Rab6效应子的小的、部分重叠的亚群对高尔基体组织和功能所必需的两种系链依赖通路具有不同的重要性。
The organization of the Golgi apparatus is determined in part by the interaction of Rab proteins and their diverse array of effectors. Here, we used multiple approaches to identify and characterize a small subset of effectors that mimicked the effects of Rab6 on Golgi ribbon organization. In a visual-based, candidate-protein screen, we found that the individual depletion of any of three Rab6 effectors, myosin IIA (MyoIIA), Kif20A, and Bicaudal D (BicD), was sufficient to suppress Golgi ribbon fragmentation/dispersal coupled to retrograde tether proteins in a manner paralleling Rab6. MyoIIA and Kif20A depletion were pathway selective and suppressed ZW10-dependent Golgi ribbon fragmentation/dispersal only while BicD depletion, like Rab6, suppressed both ZW10- and COG-dependent Golgi ribbon fragmentation. The MyoIIA effects could be produced in short term assays by the reversible myosin inhibitor, blebbistatin. At the electron microscope level, the effects of BicD-depletion mimicked many of those of Rab6-depletion: longer and more continuous Golgi cisternae and a pronounced accumulation of coated vesicles. Functionally, BicD-depleted cells were inhibited in transport of newly synthesized VSV-G protein to the cell surface. In sum, our results indicate small, partially overlapping subsets of Rab6 effectors are differentially important to two tether-dependent pathways essential to Golgi organization and function.
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影响因子：
21.3
作者：
Miserey-Lenkei, S.;Chalancon, G.;Echard, A.
通讯作者：
Echard, A.
DOI：
10.1073/pnas.94.5.1828
发表时间：
1997-03-04
影响因子：
11.1
作者：
Martinez, O;Antony, C;Goud, B
通讯作者：
Goud, B
影响因子：
4.5
作者：
Shestakova, A;Zolov, S;Lupashin, V
通讯作者：
Lupashin, V
DOI：
10.1073/pnas.051631998
发表时间：
2001-02-27
影响因子：
11.1
作者：
Marsh, BJ;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
11.8
作者：
Grigoriev, Ilya;Splinter, Daniel;Akhmanova, Anna
通讯作者：
Akhmanova, Anna