Searching for biomarkers: humoral response profiling with luciferase immunoprecipitation systems.
Searching for biomarkers: humoral response profiling with luciferase immunoprecipitation systems.
复制标题
搜索生物标志物：使用荧光素酶免疫沉淀系统进行体液反应分析。
DOI：
10.1586/epr.11.23
复制
发表时间：
2011-06
影响因子：
3.4
通讯作者：
Iadarola MJ
中科院分区：
文献类型：
--
作者：
Burbelo PD;Ching KH;Bren KE;Iadarola MJ
B细胞介导的体液反应在许多人类疾病中被触发，包括自身免疫性疾病、癌症、神经系统疾病和传染病。然而，由于许多免疫测定形式的诊断性能较差，充分利用患者抗体库中包含的信息进行诊断、监测甚至疾病预测受到限制。我们开发了荧光素酶免疫沉淀系统（LIPS），利用发光蛋白质生成最适合诊断和生物标志物发现的高清晰度抗体谱。在这里，我们描述了在我们的实验室进行的一系列LIPS抗体谱研究的结果和影响。其中包括对国内和全球病原体的高灵敏度诊断，对感染相关疾病的洞察，发现人类疾病的新生物标志物，症状的亚类分类和针对自身蛋白质的致病性自身抗体的鉴定。这些研究突出了与不同疾病相关的体液反应谱的类型，提供了与疾病发病机制相关的新信息，并为将LIPS抗体谱纳入全球卫生倡议和疾病监测提供了框架。
B-cell mediated humoral responses are triggered in many human diseases including autoimmune, cancer, neurologic, and infectious diseases. However, the full exploitation of the information contained within a patient's antibody repertoire, for diagnosis, monitoring and even disease prediction has been limited due to the poor diagnostic performance of many immunoassay formats. We have developed Luciferase immunoprecipitation systems (LIPS) that harnesses light emitting proteins to generate high definition antibody profiles optimal for both diagnostics and biomarker discovery. Here we describe the results and implications from a range of LIPS antibody profiling studies performed in our laboratory. These include highly sensitive diagnostics for domestic and global pathogens, insights into infection-related diseases, discovery of new biomarkers for human diseases, subcategorization of symptoms and identification of pathogenic autoantibodies against self-proteins. These investigations highlight the types of humoral response profiles associated with different diseases, provide new information related to disease pathogenesis, and provide a framework for incorporating LIPS antibody profiling into global health initiatives and disease monitoring.
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影响因子：
7.4
作者：
Burbelo PD;Seam N;Groot S;Ching KH;Han BL;Meduri GU;Iadarola MJ;Suffredini AF
通讯作者：
Suffredini AF
用于自身抗体的新发光测定法对哺乳动物预制作的胰岛素瘤相关蛋白2。
影响因子：
16.2
作者：
Burbelo, Peter D.;Hirai, Hiroki;Leahy, Hannah;Lernmark, Ake;Ivarsson, Sten A.;Iadarola, Michael J.;Notkins, Abner Louis
通讯作者：
Notkins, Abner Louis
影响因子：
16.2
作者：
Burbelo PD;Hirai H;Issa AT;Kingman A;Lernmark A;Ivarsson SA;Notkins AL;Iadarola MJ
通讯作者：
Iadarola MJ
影响因子：
20.3
作者：
Cohen, Jeffrey I.;Jaffe, Elaine S.;Straus, Stephen E.
通讯作者：
Straus, Stephen E.
影响因子：
6.4
作者：
Burbelo, Peter D.;Kovacs, Joseph A.;Kottilil, Shyam
通讯作者：
Kottilil, Shyam